Genetic Variability among flavescence dorie phytoplasmas from different origins in Italy and France (Articolo in rivista)

Type
Label
  • Genetic Variability among flavescence dorie phytoplasmas from different origins in Italy and France (Articolo in rivista) (literal)
Anno
  • 2002-01-01T00:00:00+01:00 (literal)
Alternative label
  • Martini M., Botti S., Marcone C., Marzachl C., Casati P., Bianco Pa., Benedetti R., Bertaccini A. (2002)
    Genetic Variability among flavescence dorie phytoplasmas from different origins in Italy and France
    in Molecular and cellular probes (Print)
    (literal)
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#autori
  • Martini M., Botti S., Marcone C., Marzachl C., Casati P., Bianco Pa., Benedetti R., Bertaccini A. (literal)
Pagina inizio
  • 197 (literal)
Pagina fine
  • 208 (literal)
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#numeroVolume
  • 16 (literal)
Rivista
Note
  • ISI Web of Science (WOS) (literal)
Titolo
  • Genetic Variability among flavescence dorie phytoplasmas from different origins in Italy and France (literal)
Abstract
  • Flavescence doree is a devastating disease of grapevine widespread in several countries in EU such as France, Italy and Spain. Genetic variability among 17 Italian and 3 French FD strains was investigated by RFLP analyses based on a fragment of the ribosomal protein operon and on the non-ribosomal DNA fragment FD9. RFLP analysis of the PCR amplified ribosomal protein fragment/ coding for the 3' end of rpl22 and the entire rps3 genes, differentiated 4 rp-subgroups among the FD strains and 4 subgroups among the reference strains belonging to elm yellows group (16SrV). Sequencing and phylogenetic analysis of the same ribosomal protein DNA fragment validated the delineation of 4 distinct FD strain types derived by RFLP analyses. The results supported the differentiation based on analysis of the non-ribosomal DNA fragment FD9. The phylogenetic analysis further revealed relationships and a probable evolutionary trend among the FD strains and the other representatives of elm yellows group. All the FD strains together with the reference strains ALY, RuS and JWB formed a cluster very well distinct from the EY/ULW cluster. Moreover, ALY was shown to be more closely related to three FD strain types: the Lombardia/Piemonte, the French FD70, and the French FD88/ltalian FD-D strain clusters. (literal)

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