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First report of phytoplasmas infecting swan plants (Gomphocarpus physocarpus) in Liguria, Italy (Articolo in rivista)
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- First report of phytoplasmas infecting swan plants (Gomphocarpus physocarpus) in Liguria, Italy (Articolo in rivista) (literal)
- Anno
- 2002-01-01T00:00:00+01:00 (literal)
- Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#doi
- 10.1046/j.1365-3059.2002.00766.x (literal)
- Alternative label
D'Aquilio M., Boarino A., Bozzano G., Marzachì C., Roggero P., Boccardo, G. (2002)
First report of phytoplasmas infecting swan plants (Gomphocarpus physocarpus) in Liguria, Italy
in Plant pathology (Print); Wiley-Blackwell, Oxford (Regno Unito)
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- D'Aquilio M., Boarino A., Bozzano G., Marzachì C., Roggero P., Boccardo, G. (literal)
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- (New Disease Reports) (literal)
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- ISI Web of Science (WOS) (literal)
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- dAM, BA, MC, RP, BG: Istituto di Virologia Vegetale, CNR, Torino, Italia
BG: Cooperativa l'Ortofrutticola, Albenga (SV), Italia (literal)
- Titolo
- First report of phytoplasmas infecting swan plants (Gomphocarpus physocarpus) in Liguria, Italy (literal)
- Abstract
- Swan plants [Gomphocarpus physocarpus E. Mey. (=Asclepias physocarpa L.);
Asclepiadaceae are deciduous subshrubs grown in the open in the Albenga
area of the Italian Riviera. During summer 2001, several plants in a local
field (field A) showed witches-broom, dwarfing, yellowing and decline
symptoms with total loss of normal flower production on one plant (Fig.
1). Witches-brooms, floral virescence and phyllody were all symptoms
observed on 60-70% of plants (Fig. 2) in a second field (field B). No
virus particles were observed by transmission electron microscopy (TEM) in
negatively stained preparations from symptomatic plants nor were viruses
isolated from several herbaceous hosts after mechanical inoculation of
these hosts with preparations from diseased swan plants. Total nucleic
acids were extracted from tissues (Marzachì et al., 2000) of both
symptomatic (five plants from field A and four field B) and asymptomatic
plants (one from each field). Resulting extracts were analysed by a
polymerase chain reaction (PCR) assay employing universal rRNA primer pair
P1/P7 (Schneider et al., 1995). A 1.8 kbp rDNA product was generated
exclusively from all symptomatic plants.
Restriction fragment length polymorphism (RFLP) patterns after
digestion of P1/P7-primed products with endonuclease MseI (Fig. 3)
indicated that all five symptomatic plants analysed from field A contained
a phytoplasma indistinguishable from stolbur (Stol) phytoplasma, an
established 16SrDNA RFLP group (16Sr) subgroup A (16SrXII-A) strain. By
comparison, four plants from field B all contained phytoplasmas
indistinguishable from European aster yellows, a phytoplasma previously
classified as a 16SrI-B subgroup strain. Using these same assay methods,
Stol phytoplasma was also detected in symptomatic Alstroemeria sp. and
Solanum nigrum L. plants collected near field A.
Phytoplasmas have been previously observed by TEM in phloem sieve elements
of Gomphocarpus sp. with yellowing symptoms in the Brazilian State of São
Paolo (Kitajima and Costa, 1979) whereas in the north-eastern USA, dwarfed
and chlorotic Asclepias syriaca L. (=A. fruticosa L., =Gomphocarpus
fruticosus R. Br.) reportedly contained 16SrIII group phytoplasmas
(Griffiths et al.1994). This is the first record of phytoplasmas infecting
members of the Asclepiadaceae family in the Old World. (literal)
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