http://www.cnr.it/ontology/cnr/individuo/prodotto/ID56578
DNA-based methods for the detection and the identification of phytoplasmas in insect vector extracts (Articolo in rivista)
- Type
- Label
- DNA-based methods for the detection and the identification of phytoplasmas in insect vector extracts (Articolo in rivista) (literal)
- Anno
- 2002-01-01T00:00:00+01:00 (literal)
- Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#doi
- 10.1385/MB:22:1:009 (literal)
- Alternative label
Bosco D., Palermo S., Mason G., Tedeschi R., Marzachl C., Boccardo G. (2002)
DNA-based methods for the detection and the identification of phytoplasmas in insect vector extracts
in Molecular biotechnology; Humana Press Inc., Totowa (Stati Uniti d'America)
(literal)
- Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#autori
- Bosco D., Palermo S., Mason G., Tedeschi R., Marzachl C., Boccardo G. (literal)
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- Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#numeroFascicolo
- Note
- ISI Web of Science (WOS) (literal)
- Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#affiliazioni
- MC, BG: Istituto di Virologia Vegetale, CNR, Torino, Italia
BD, PS, MG, TR: Università di Torino, Italia (literal)
- Titolo
- DNA-based methods for the detection and the identification of phytoplasmas in insect vector extracts (literal)
- Abstract
- DNA extraction and storage methods have been evaluated using laboratory-
reared leafhoppers and/or field-collected leafhoppers and psyllids.
Detection of four different phytopathogenic phytoplasmas, belonging to
three taxonomic groups, has been achieved by several direct or nested
polymerase chain reaction methods with such DNA extracts. Reactions
differed in both 16/23S ribosomal primer pairs used and specific assay and
cycling conditions. Merits and possible hindrances of the various primer
pairs, in relation to insect DNA extracts, are discussed. However,
identification of the phytoplasma(s), necessarily relied on the comparison
of the length polymorphism of the amplified DNA fragments obtained by
restriction with appropriate endonucleases. Endonuclease digestion is
crucial for determining the identity (=subgroup affiliation) of
phytoplasmas of the same groups that can be carried by one individual
vector. (literal)
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