Cryopreservation of redwood (Sequoia sempervirens (D. Don.) Endl.) in vitro buds using vitrification-based techniques” (Articolo in rivista)

Type
Label
  • Cryopreservation of redwood (Sequoia sempervirens (D. Don.) Endl.) in vitro buds using vitrification-based techniques” (Articolo in rivista) (literal)
Anno
  • 2011-01-01T00:00:00+01:00 (literal)
Alternative label
  • Ozudogru E.A.; Kirdok E.; Kaya E.; Capuana M.; Benelli C.; Engelmann F. (2011)
    Cryopreservation of redwood (Sequoia sempervirens (D. Don.) Endl.) in vitro buds using vitrification-based techniques”
    in Cryo-letters; CRYO LETTERS, C/O ROYAL VETERINARY COLLEGE, ROYAL COLLEGE ST, LONDON NW1 0TU, ENGLAND, London (Regno Unito)
    (literal)
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#autori
  • Ozudogru E.A.; Kirdok E.; Kaya E.; Capuana M.; Benelli C.; Engelmann F. (literal)
Pagina inizio
  • 99 (literal)
Pagina fine
  • 110 (literal)
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#url
  • http://www.cryoletters.org/index.htm (literal)
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#numeroVolume
  • 32 (literal)
Rivista
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#note
  • Issue: 2 (literal)
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#pagineTotali
  • 12 (literal)
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#numeroFascicolo
  • 2 (literal)
Note
  • ISI Web of Science (WOS) (literal)
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#affiliazioni
  • Gebze Institute of Technology, Faculty of Science, Department of Molecular Biology and Genetics, Istanbul Caddesi, No 101, 41400 Gebze (Kocaeli), Turkey. IGV Istituto di Genetica Vegetale, via Madonna del Piano 10 50019 Sesto Fiiorentino (FI). IVALSA Istituto per la Valorizzazione del Legno e delle Specie Arboree, via Madonna del Piano 10 50019 Sesto Fiorentino (FI). IRD/Institut de Recherche pour le Développement, UMR DIAPC, 911 avenue Agropolis, BP 64501, 34394 Montpellier Cedex 5, France, Bioversity International, Via dei Tre Denari 472/a, 00057 Maccarese (Fiumicino), Rome, Italy. (literal)
Titolo
  • Cryopreservation of redwood (Sequoia sempervirens (D. Don.) Endl.) in vitro buds using vitrification-based techniques” (literal)
Abstract
  • In this study, the efficiency of three vitrification-based cryopreservation techniques, i.e. vitrification, encapsulation-vitrification and droplet-vitrification were compared for cryopreserving Sequoia sempervirens apical and basal buds sampled from in vitro shoot cultures. The effect of cold-hardening of mother-plants and of bud culture medium and sucrose preculture was also investigated. Culture of apical and basal buds sampled from coldhardened mother-plants on Quoirin and Lepoivre medium with activated charcoal had a positive effect on regrowth. Only droplet-vitrification ensured survival and regrowth after cryopreservation. After cryopreservation, regeneration of apical buds was possible for PVS2 exposure durations between 90 and 180 min but it remained low, with a maximum of 18% after 135 min treatment. With basal buds, regeneration after cryopreservation was possible over a larger range of PVS2 treatment durations, between 30 and 180 min. The highest regeneration percentage was slightly higher (22%) than that measured with apical buds, and was also achieved after 135 min PVS2 exposure. (literal)
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