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Cryopreservation of Pistacia spp. seeds by dehydration and one-step freezing (Articolo in rivista)

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Cryopreservation of Pistacia spp. seeds by dehydration and one-step freezing (Articolo in rivista) (literal)

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Y. Ozden-Tokatli ; E.A. Ozudogru ; F. Gumusel ; M. Lambardi (2007)
Cryopreservation of Pistacia spp. seeds by dehydration and one-step freezing
in Cryo-letters
(literal)

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Gebze Institute of Technology, Faculty of Science, Department of Biology. Istanbul cad. No: 101, 41400, Gebze, Kocaeli, Turkey ; Gebze Institute of Technology, Faculty of Science, Department of Biology. Istanbul cad. No: 101, 41400, Gebze, Kocaeli, Turkey ; Gebze Institute of Technology, Faculty of Science, Department of Biology. Istanbul cad. No: 101, 41400, Gebze, Kocaeli, Turkey ; IVALSA/Istituto per la Valorizzazione del Legno e delle Specie Arboree, CNR/Consiglio Nazionale delle Ricerche, Polo Scientifico, 50019 Sesto Fiorentino (Firenze), Italy (literal)

Titolo

Cryopreservation of Pistacia spp. seeds by dehydration and one-step freezing (literal)

Abstract

Cryopreservation protocols by dehydration and one-step freezing were developed for seeds from three Pistacia species, i.e., P. vera, P. terebinthus and P. lentiscus, which were characterised. by different initial germination percentages (100%, 17% and 81%, respectively). In P. vera, a maximum of 90% germination was obtained following 8 hours drying in silica gel (corresponding to 11.7% moisture content on a FW basis) and direct immersion in LN. In P. terebinthus and P. lentiscus, shorter periods of dehydration (I hour and 15 min, respectively) were sufficient to reduce their moisture content to about 20%, which resulted in peak seed germination percentages from cryostorage of 16% and 47%, respectively. Following cryopreservation, the seeds germinated better on semi-solid MS medium, than on cotton wool wetted with dH(2)O or liquid MS medium. Finally, in P. vera and P. lentiscus, high and significant correlation coefficients were obtained between the TTC viability test and seed germinability after recovery from LN, provided that seeds which were considered positive in the test showed completely or partially red embryonic axes coupled to completely red cotyledons. (literal)

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