Anchoring of unmodified oligonucleotides on a chitosan-based array: applications to genotyping and gene expression (Articolo in rivista)

Type
Label
  • Anchoring of unmodified oligonucleotides on a chitosan-based array: applications to genotyping and gene expression (Articolo in rivista) (literal)
Anno
  • 2008-01-01T00:00:00+01:00 (literal)
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#doi
  • 10.1016/j.ab.2008.01.031 (literal)
Alternative label
  • Clarissa Consolandi; Marco Severgnini; Giada Caredda; Gianluca De Bellis (2008)
    Anchoring of unmodified oligonucleotides on a chitosan-based array: applications to genotyping and gene expression
    in Analytical biochemistry (Print)
    (literal)
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#autori
  • Clarissa Consolandi; Marco Severgnini; Giada Caredda; Gianluca De Bellis (literal)
Pagina inizio
  • 39 (literal)
Pagina fine
  • 43 (literal)
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#numeroVolume
  • 376 (literal)
Rivista
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#note
  • May 1;376(1):39-43. Epub 2008 Jan 31. (literal)
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#numeroFascicolo
  • 1 (literal)
Note
  • ISI Web of Science (WOS) (literal)
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#affiliazioni
  • CNR ITB (literal)
Titolo
  • Anchoring of unmodified oligonucleotides on a chitosan-based array: applications to genotyping and gene expression (literal)
Abstract
  • Oligonucleotide arrays are one of the most used technologies in genotyping and gene expression experiments. Here, a previously developed chitosan-based platform is characterized in its binding of unmodified oligonucleotides, increasing the cost effectiveness of the microarray production process. The unmodified oligonucleotides on the activated chitosan surface showed the same or better performances than amino-modified probes. Moreover, we show applications in genotyping (a ligation detection reaction experiment on cyanobacteria) and in gene expression (on peach RNA samples). The platform was demonstrated to be reliable, robust, and reproducible for immobilizing unmodified oligonucleotides in high quality oligonucleotide array fabrication. (c) 2008 Elsevier Inc. All rights reserved. (literal)
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