Fractionation techniques improve the proteomic analysis of human serum (Articolo in rivista)

Type
Label
  • Fractionation techniques improve the proteomic analysis of human serum (Articolo in rivista) (literal)
Anno
  • 2008-01-01T00:00:00+01:00 (literal)
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#doi
  • 10.2174/157341208784246297 (literal)
Alternative label
  • Pierluigi Mauri, Andrea Petretto, Davide Cuccabita, Fabrizio Basilico, Dario Di Silvestre, Isabella Levreri and Giovanni Melioli (2008)
    Fractionation techniques improve the proteomic analysis of human serum
    in Current pharmaceutical analysis (Print)
    (literal)
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#autori
  • Pierluigi Mauri, Andrea Petretto, Davide Cuccabita, Fabrizio Basilico, Dario Di Silvestre, Isabella Levreri and Giovanni Melioli (literal)
Pagina inizio
  • 69 (literal)
Pagina fine
  • 77 (literal)
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#numeroVolume
  • 4 (literal)
Rivista
Note
  • ISI Web of Science (WOS) (literal)
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#affiliazioni
  • CNR - Istituto di Tecnologie biomediche (literal)
Titolo
  • Fractionation techniques improve the proteomic analysis of human serum (literal)
Abstract
  • Different fractionation steps have been developed to reduce the complexity of serum proteome and to allow the detection and the identification of single proteins. The nature of fractionation ( performed either on denatured or native proteins), the efficiency of recovery, the capacity of directly identifying proteins or protein panels, the possibility of associating to other laboratory techniques influence the choice of the methods to be used in different experimental and clinical settings. In this review, the main fractionation techniques (such as electrophoresis, SELDI and liquid chromatography) are described for proteomic studies in clinical field, and their advantages and disadvantages are discussed on the basis of our experience. In particular, liquid-liquid fractionation performed by PF2D is discussed in detail because of its partial automation, its ability to improve mass spectrometry analysis of serum proteins and its potential application in clinical studies. (literal)
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