Differential liquid phase proteomic analysis of the effect of selenium supplementation in LNCaP cells. (Articolo in rivista)

Type

• Prodotto della ricerca (Classe)
• Articolo in rivista (Classe)

Label

• Differential liquid phase proteomic analysis of the effect of selenium supplementation in LNCaP cells. (Articolo in rivista) (literal)

Anno

• 2008-01-01T00:00:00+01:00 (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#doi

• 10.1016/j.jchromb.2008.02.010 (literal)

Alternative label

• Roveri A., Vitale MP, Serain E, Zaccarin M, Mauri P, Di Silvestre D, De Palma A, Gion M, Toppo S, Maiorino M, Ursini F. (2008)
  Differential liquid phase proteomic analysis of the effect of selenium supplementation in LNCaP cells.
  in Journal of chromatography. B (Print); ELSEVIER SCIENCE BV, PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS, AMSTERDAM (Paesi Bassi)
  (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#autori

• Roveri A., Vitale MP, Serain E, Zaccarin M, Mauri P, Di Silvestre D, De Palma A, Gion M, Toppo S, Maiorino M, Ursini F. (literal)

Pagina inizio

• 63 (literal)

Pagina fine

• 73 (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#url

• http://www.sciencedirect.com/science/article/pii/S157002320800127X (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#numeroVolume

• 865 (literal)

Rivista

• Journal of chromatography. B (Rivista)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#numeroFascicolo

• 1-2 (literal)

Note

• Scopu (literal)
• ISI Web of Science (WOS) (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#affiliazioni

• Univ Padua, Dept Biol Chem, I-35131 Padua, Italy ; Reg Hosp, Consortium Ist Oncol Veneto IRCCS, Ctr Study Biol Markers Malignancy, Venice, Italy ; IRCCS, IOV, Padua, Italy ; CNR, ITB, I-20133 Milan, Italy (literal)

Titolo

• Differential liquid phase proteomic analysis of the effect of selenium supplementation in LNCaP cells. (literal)

Abstract

• The effect of 100 nM sodium selenite supplementation was studied on LNCaP cells by a proteomic approach, on ProteomeLab (TM) PF 2D platform. Proteins were separated by liquid phase bi-dimensional chromatography and analyzed by pair-wise alignment of peaks to detect those differentially expressed. Differential expression threshold was set at a twice difference level and proteins matching this criterion were identified by MALDI-TOF and confirmed by ESI-ion trap MS/MS. Not all differentially expressed proteins found by PF 2D could be identified by MS analysis, the sensitivity of which emerging as the limiting factor. Thus, only the most abundant proteins, differently expressed following selenium supplementation, were identified. We positively showed an increase of expression of thioredoxin reductase 1, enolase 1, phosphoglycerate mutase 1, glyceraldehyde3-phosphate dehydrogenase, heterogeneous nuclear ribonucleoprotein A2/B1, isoform A2, Ras-GTPase-activating protein SH3-domain-binding protein and Keratin 18 and a decrease of expression of peroxiredoxin I and heat shock protein 70, protein 8, isofonn 1. Results are consistent, at least in part, with the less oxidant environment brought about by the synthesis of Se-dependent peroxidases, keeping low the steady-state concentration of hydrogen peroxide. (c) 2008 Elsevier B.V. All rights reserved. (literal)

Editore

• ELSEVIER SCIENCE BV, PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS (Editore)

Prodotto di

• ProteoMetabolomica - Sviluppo ed applicazione della LC-MS/MS per lo studio di proteine e metaboliti (PM.P06.008.003) (Modulo)

Autore CNR

• PIETRO LUIGI MAURI (Unità di personale interno)
• DARIO DI SILVESTRE (Persona)
• ANTONELLA DE PALMA (Unità di personale interno)

Insieme di parole chiave

• Parole chiave di "Differential liquid phase proteomic analysis of the effect of selenium supplementation in LNCaP cells." (Insieme di parole chiave)

Incoming links:

Autore CNR di

• PIETRO LUIGI MAURI (Unità di personale interno)
• DARIO DI SILVESTRE (Persona)
• ANTONELLA DE PALMA (Unità di personale interno)

Prodotto

• ProteoMetabolomica - Sviluppo ed applicazione della LC-MS/MS per lo studio di proteine e metaboliti (PM.P06.008.003) (Modulo)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#rivistaDi

• Journal of chromatography. B (Rivista)

Editore di

• ELSEVIER SCIENCE BV, PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS (Editore)

Insieme di parole chiave di

• Parole chiave di "Differential liquid phase proteomic analysis of the effect of selenium supplementation in LNCaP cells." (Insieme di parole chiave)