New approach for rapid detection of known hemoglobin variants using LC-MS/MS combined with a peptide database (Articolo in rivista)

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  • New approach for rapid detection of known hemoglobin variants using LC-MS/MS combined with a peptide database (Articolo in rivista) (literal)
Anno
  • 2007-01-01T00:00:00+01:00 (literal)
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#doi
  • 10.1002/jms.1160 (literal)
Alternative label
  • F. Basilico, D. Di Silvestre, S. Sedini, A. Petretto, I. Levreri, G. Melioli, C. Farina, F. Mori and P. L. Mauri (2007)
    New approach for rapid detection of known hemoglobin variants using LC-MS/MS combined with a peptide database
    in Journal of mass spectrometry (Print); John Wiley & Sons, Ltd., Chichester (Regno Unito), Chichester (Regno Unito)
    (literal)
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#autori
  • F. Basilico, D. Di Silvestre, S. Sedini, A. Petretto, I. Levreri, G. Melioli, C. Farina, F. Mori and P. L. Mauri (literal)
Pagina inizio
  • 288 (literal)
Pagina fine
  • 292 (literal)
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#numeroVolume
  • 42 (literal)
Rivista
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#numeroFascicolo
  • 3 (literal)
Note
  • ISI Web of Science (WOS) (literal)
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#affiliazioni
  • ITB-CNR ; Ospedale Gaslini (GE) ; Kedrion S.p.A. (literal)
Titolo
  • New approach for rapid detection of known hemoglobin variants using LC-MS/MS combined with a peptide database (literal)
Abstract
  • The identification of hemoglobin (Hb) variants is usually performed by means of different analytical steps and methodologies. Phenotypic methods, such as gel electrophoresis and high performance liquid chromatography, are used to detect the different electrophoretic or chromatographic behaviors of hemoglobin variants in comparison to HbA(0) used as a control. These data often need to be combined with mass spectrometry analyses of intact globins and their tryptic peptide mixtures. As an alternative to a 'step-by-step' procedure, we have developed a 'single step' approach for the identification of Hb variants present in biological samples. This is based on the mu HPLC-ESl-MS/MS analysis of the peptide mixture generated by a tryptic digestion of diluted Hb samples and an in-house new database containing solely the variant tryptic peptide of known human Hb variants. The experimental results (full MS and MS/MS spectra) are correlated with theoretical mass spectra generated from our in-house-built variant peptide database (Hbp) using the SEQUEST algorithm. Simple preparation of samples and an automated identification of the variant peptide are the main characteristics of this approach, making it an attractive method for the detection of Hb variants at the routine clinical level. We have analyzed 16 different samples, each containing a different known variant of hemoglobin. (literal)
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