Complete gene expression profiling of Saccharopolyspora erythraea using GeneChip DNA microarrays. (Articolo in rivista)

Type
Label
  • Complete gene expression profiling of Saccharopolyspora erythraea using GeneChip DNA microarrays. (Articolo in rivista) (literal)
Anno
  • 2007-01-01T00:00:00+01:00 (literal)
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#doi
  • 10.1186/1475-2859-6-37 (literal)
Alternative label
  • Peano C, Bicciato S, Corti G, Ferrari F, Rizzi E, Bonnal RJ, Bordoni R, Albertini A, Bernardi LR, Donadio S, De Bellis G. (2007)
    Complete gene expression profiling of Saccharopolyspora erythraea using GeneChip DNA microarrays.
    in Microbial cell factories
    (literal)
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#autori
  • Peano C, Bicciato S, Corti G, Ferrari F, Rizzi E, Bonnal RJ, Bordoni R, Albertini A, Bernardi LR, Donadio S, De Bellis G. (literal)
Pagina inizio
  • 37-1 (literal)
Pagina fine
  • 37-15 (literal)
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#numeroVolume
  • 6 (literal)
Rivista
Note
  • ISI Web of Science (WOS) (literal)
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#affiliazioni
  • 1. CNR, Inst Biomed Technol, Milan, Italy 2. Univ Padua, Dept Chem Proc Engn, Padua, Italy 3. Univ Modena, Dept Chem Proc Engn, Modena, Italy 4. Univ Reggio Emilia, Dept Chem Proc Engn, Modena, Italy 5. Univ Milan, Dept Biomed Sci & Technol, Milan, Italy 6. KtedoGen, Milan, Italy (literal)
Titolo
  • Complete gene expression profiling of Saccharopolyspora erythraea using GeneChip DNA microarrays. (literal)
Abstract
  • ABSTRACT: BACKGROUND: The Saccharopolyspora erythraea genome sequence, recently published, presents considerable divergence from those of streptomycetes in gene organization and function, confirming the remarkable potential of S. erythraea for producing many other secondary metabolites in addition to erythromycin. In order to investigate, at whole transcriptome level, how S. erythraea genes are modulated, a DNA microarray was specifically designed and constructed on the S. erythraea strain NRRL 2338 genome sequence, and the expression profiles of 6494 ORFs were monitored during growth in complex liquid medium. RESULTS: The transcriptional analysis identified a set of 404 genes, whose transcriptional signals vary during growth and characterize three distinct phases: a rapid growth until 32 h (Phase A); a growth slowdown until 52 h (Phase B); and another rapid growth phase from 56 h to 72 h (Phase C) before the cells enter the stationary phase. A non-parametric statistical method, that identifies chromosomal regions with transcriptional imbalances, determined regional organization of transcription along the chromosome, highlighting differences between core and non-core regions, and s (literal)
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