http://www.cnr.it/ontology/cnr/individuo/prodotto/ID55870
Analysis of 10B antitumoral compounds by means of flow-injection into ESI-MS/MS (Articolo in rivista)
- Type
- Label
- Analysis of 10B antitumoral compounds by means of flow-injection into ESI-MS/MS (Articolo in rivista) (literal)
- Anno
- 2005-01-01T00:00:00+01:00 (literal)
- Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#doi
- 10.1002/jms.909 (literal)
- Alternative label
- Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#autori
- F. Basilico; W. Sauerwein; F. Pozzi; A. Wittig; R. Moss; P. L. Mauri (literal)
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- Paper presented at the 23rd Informal Meeting on Mass Spectrometry, Fiera di Primiero, Italy, 15-19 May 2005 (literal)
- Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#numeroVolume
- Rivista
- Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#numeroFascicolo
- Note
- ISI Web of Science (WOS) (literal)
- Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#affiliazioni
- Istituto Tecnologie Biomediche
Strahlenklinik, Universitatsklinikum Essen
Nuclear Research and consultancy Group, NRG Petten, The Netherlands (literal)
- Titolo
- Analysis of 10B antitumoral compounds by means of flow-injection into ESI-MS/MS (literal)
- Abstract
- Boron neutron capture therapy (BNCT) is a promising binary treatment for cancer. BNCT is based on the ability of the nonradioactive isotope B-10 to capture, with a very high probability, thermal neutrons. This nuclear reaction results in two particles (an alpha and a lithium nucleus). The particles have a high biological effectiveness, which is limited in tissue to approximately the diameter of one cell. If the reaction can be limited to a tumor cell, the physical characteristic opens up the possibility to selectively destroy cancer cells, while sparing the surrounding healthy tissue. Quality control of B-10-containing compounds and their distribution at present are very important, and different analytical methods have been developed, such as time-of-flight secondary ion mass spectrometry (TOF-SIMS), electron energy loss spectrometry (EELS), prompt gamma analysis and inductively coupled plasma-optical emission spectrometry (ICP-OES). These methods allow the analyses of B-10, but it is not possible to characterize the specific molecular compounds containing B-10. For this reason, we propose a fast and quantitative method that permits the determination of closo-undecahydro-1-mercaptododecaborate (BSH) and (10)boron-phenylalanine (BPA) and their eventual metabolites. In particular, B-10-containing compounds are detected by means of flow-injection electrospray tandem mass spectrometry (FI/ESI-MS/MS). This approach allows the identification of Boron compounds, BSH and BPA, using tandem mass spectrometry, and quantitative analysis is also possible (c.v. +/- 4.7%; n = 5; linear range 10-10000 ng/ml). Furthermore, B-10-containing compounds were detected in actual biological sample (urine and plasma, diluted 10000- and 1000-fold, respectively) injecting a small volume (1 mu l) of diluted samples. Copyright (c) 2005 John Wiley & Sons, Ltd. (literal)
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