Application of the Taguchi method to the analysis of the deposition step in microarray production. (Articolo in rivista)

Type

• Prodotto della ricerca (Classe)
• Articolo in rivista (Classe)

Label

• Application of the Taguchi method to the analysis of the deposition step in microarray production. (Articolo in rivista) (literal)

Anno

• 2006-01-01T00:00:00+01:00 (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#doi

• 10.1109/TNB.2006.880851 (literal)

Alternative label

• Severgnini M; Pattini L; Consolandi C; Rizzi E; Battaglia C; De Bellis G; Cerutti S. (2006)
  Application of the Taguchi method to the analysis of the deposition step in microarray production.
  in IEEE transactions on nanobioscience
  (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#autori

• Severgnini M; Pattini L; Consolandi C; Rizzi E; Battaglia C; De Bellis G; Cerutti S. (literal)

Pagina inizio

• 164 (literal)

Pagina fine

• 172 (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#numeroVolume

• 5 (literal)

Rivista

• IEEE transactions on nanobioscience (Rivista)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#numeroFascicolo

• 3 (literal)

Note

• ISI Web of Science (WOS) (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#affiliazioni

• Institute of Biomedical Technologies of the National Research Council (literal)

Titolo

• Application of the Taguchi method to the analysis of the deposition step in microarray production. (literal)

Abstract

• Every microarray experiment is affected by many possible sources of variability that may even corrupt biological evidence on analyzed sequences. We applied a \"Taguchi method\" strategy, based on the use of orthogonal arrays to optimize the deposition step of oligonucleotide sequences on glass slides. We chose three critical deposition parameters (humidity, surface, and buffer) at two levels each, in order to establish optimum settings. A L-8 orthogonal array was used in order to monitor both the main effects and interactions on the deposition of a 25 mer oligonucleotide hybridized to its fluorescent-labeled complementary. Signal-background ratio and deposition homogeneity in terms of mean intensity and spot diameter were considered as significant outputs. An analysis of variance (ANOVA) was applied to raw data and to mean results for each slide and experimental run. Finally we calculated an overall evaluation coefficient to group together important outputs in one number. Environmental humidity and surface-buffer interaction were recognized as the most critical factors, for which a 50% humidity, associated to a chitosan-covered slide and a sodium phosphate + 25% dimethyl sulfoxide (DMSO) buffer gave best performances. Our results also suggested that Taguchi methods can be efficiently applied in optimization of microarray procedures. (literal)

Prodotto di

• Sviluppo di un cyberspazio per l'alta formazione e le tecnologie didattiche (PM.P06.009.002) (Modulo)
• Tecnologie ad Array e di sequenziamento massivo (PM.P06.009.001) (Modulo)

Autore CNR

• MARCO SEVERGNINI (Unità di personale esterno)
• ERMANNO RIZZI (Persona)
• GIANLUCA DE BELLIS (Persona)
• CLARISSA CONSOLANDI (Persona)

Incoming links:

Autore CNR di

• CLARISSA CONSOLANDI (Persona)
• GIANLUCA DE BELLIS (Persona)
• ERMANNO RIZZI (Persona)
• MARCO SEVERGNINI (Unità di personale esterno)

Prodotto

• Tecnologie ad Array e di sequenziamento massivo (PM.P06.009.001) (Modulo)
• Sviluppo di un cyberspazio per l'alta formazione e le tecnologie didattiche (PM.P06.009.002) (Modulo)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#rivistaDi

• IEEE transactions on nanobioscience (Rivista)