Investigation of the effectc of aluminium binding in Al(III)-treated neuroblastoma cells (Articolo in rivista)

Type
Label
  • Investigation of the effectc of aluminium binding in Al(III)-treated neuroblastoma cells (Articolo in rivista) (literal)
Anno
  • 2003-01-01T00:00:00+01:00 (literal)
Alternative label
  • 148. Polec-Pawlak K., Zambenedetti P., Szpunar J., Lobinsky R., Zatta P. (2003)
    Investigation of the effectc of aluminium binding in Al(III)-treated neuroblastoma cells
    in Archives of biochemistry and biophysics (Print)
    (literal)
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  • 148. Polec-Pawlak K., Zambenedetti P., Szpunar J., Lobinsky R., Zatta P. (literal)
Pagina inizio
  • 59 (literal)
Pagina fine
  • 64 (literal)
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  • 417 (literal)
Rivista
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  • La ricerca riportata descrive la produzione di una proteine consequentemente al trattamento di cellule di neuroblastoma murino di ratto. Il lavoro √® di particolare interesse per la comprensione dei meccanismi molecolari sulla tossicit√† dell'alluminio. (literal)
Note
  • ISI Web of Science (WOS) (literal)
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#affiliazioni
  • 1. Dipartimento di Chimica Amnalitica, Universit√† di Varsavia, Polonia 2. Banca degli Encefali dell'Ospedale Civile di Dolo, Venezia 3. CNRS UMR 5034 4. CNR-ITB, sezione di Padova (literal)
Titolo
  • Investigation of the effectc of aluminium binding in Al(III)-treated neuroblastoma cells (literal)
Abstract
  • Different hyphenated techniques have been employed for the first time for a comparative stuydiy of control and metal-exposed cell lines, one of the important research areas in the field of metallomics. Speciation of aluminum (an element implicated in a variety of neurological disorders) was investigated in neuroblastoma cells exposed to aluminum lactate by size exclusion high-performance liquid chromatography inductively coupled plasma mass spectrometry (SE-HPLC-ICP-MS) and capillary electrophoresis (CE)-ICP-MS. Whereas in control cells the most intense Al compound co-eluted with the Al-transferrin complex, is size-exclusion chromatography, in exposed cells a low molecular weight bioligand was synthesized. The latter bound all the Al(III) metabolised by exposed cells in the form of a negatively-charged complex. This complex turned out to be more stable than the protein complex of Al(III) in control cells. The isolated low molecular weight Al species did not produce a signal in electrospray mass spectrometry (ES-MS) and its behaviour investigated by size- exclusion , reversed phase HPLC and CE excluded its being hydroxide, citrate, phosphate or residual lactate from the culture medium. (literal)
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