http://www.cnr.it/ontology/cnr/individuo/prodotto/ID55708
Investigation of the effectc of aluminium binding in Al(III)-treated neuroblastoma cells (Articolo in rivista)
- Type
- Label
- Investigation of the effectc of aluminium binding in Al(III)-treated neuroblastoma cells (Articolo in rivista) (literal)
- Anno
- 2003-01-01T00:00:00+01:00 (literal)
- Alternative label
148. Polec-Pawlak K., Zambenedetti P., Szpunar J., Lobinsky R., Zatta P. (2003)
Investigation of the effectc of aluminium binding in Al(III)-treated neuroblastoma cells
in Archives of biochemistry and biophysics (Print)
(literal)
- Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#autori
- 148. Polec-Pawlak K., Zambenedetti P., Szpunar J., Lobinsky R., Zatta P. (literal)
- Pagina inizio
- Pagina fine
- Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#numeroVolume
- Rivista
- Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#descrizioneSinteticaDelProdotto
- La ricerca riportata descrive la produzione di una proteine consequentemente al trattamento di cellule di neuroblastoma murino di ratto. Il lavoro è di particolare interesse per la comprensione dei meccanismi molecolari sulla tossicità dell'alluminio. (literal)
- Note
- ISI Web of Science (WOS) (literal)
- Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#affiliazioni
- 1. Dipartimento di Chimica Amnalitica, Università di Varsavia, Polonia
2. Banca degli Encefali dell'Ospedale Civile di Dolo, Venezia
3. CNRS UMR 5034
4. CNR-ITB, sezione di Padova (literal)
- Titolo
- Investigation of the effectc of aluminium binding in Al(III)-treated neuroblastoma cells (literal)
- Abstract
- Different hyphenated techniques have been employed for the first time for a comparative stuydiy of control and metal-exposed cell lines, one of the important research areas in the field of metallomics. Speciation of aluminum (an element implicated in a variety of neurological disorders) was investigated in neuroblastoma cells exposed to aluminum lactate by size exclusion high-performance liquid chromatography inductively coupled plasma mass spectrometry (SE-HPLC-ICP-MS) and capillary electrophoresis (CE)-ICP-MS. Whereas in control cells the most intense Al compound co-eluted with the Al-transferrin complex, is size-exclusion chromatography, in exposed cells a low molecular weight bioligand was synthesized. The latter bound all the Al(III) metabolised by exposed cells in the form of a negatively-charged complex. This complex turned out to be more stable than the protein complex of Al(III) in control cells. The isolated low molecular weight Al species did not produce a signal in electrospray mass spectrometry (ES-MS) and its behaviour investigated by size- exclusion , reversed phase HPLC and CE excluded its being hydroxide, citrate, phosphate or residual lactate from the culture medium. (literal)
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