http://www.cnr.it/ontology/cnr/individuo/prodotto/ID55175

Human hepatocytes and endothelial cells in organotypic membrane systems (Articolo in rivista)

Type

Prodotto della ricerca (Classe)
Articolo in rivista (Classe)

Label

Human hepatocytes and endothelial cells in organotypic membrane systems (Articolo in rivista) (literal)

Anno

2011-01-01T00:00:00+01:00 (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#doi

10.1016/j.biomaterials.2011.08.004 (literal)

Alternative label

Simona Salerno a, Carla Campana a, Sabrina Morelli a, Enrico Drioli a,b, Loredana De Bartolo a,* (2011)
Human hepatocytes and endothelial cells in organotypic membrane systems
in Biomaterials
(literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#autori

Simona Salerno a, Carla Campana a, Sabrina Morelli a, Enrico Drioli a,b, Loredana De Bartolo a,* (literal)

Pagina inizio

8848 (literal)

Pagina fine

8859 (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#url

http://www.sciencedirect.com/science/article/pii/S0142961211009069 (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#numeroVolume

32 (literal)

Rivista

Biomaterials (Rivista)

Note

ISI Web of Science (WOS) (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#affiliazioni

a Institute on Membrane Technology, National Research Council of Italy, ITM-CNR, c/o University of Calabria, via P. Bucci, Cubo 17/C, I-87030 Rende (CS), Italy b Department of Chemical Engineering and Materials, University of Calabria, via P. Bucci, Cubo 44A, 87030 Rende (CS), Italy (literal)

Titolo

Human hepatocytes and endothelial cells in organotypic membrane systems (literal)

Abstract

The realization of organotypic liver model that exhibits stable phenotype is a major challenge in the field of liver tissue engineering. In this study we developed liver organotypic co-culture systems by using synthetic and biodegradable membranes with primary human hepatocytes and human umbilical vein endothelial cells (HUVEC). Synthetic membranes prepared by a polymeric blend constituted of modified polyetheretherketone (PEEK-WC) and polyurethane (PU) and biodegradable chitosan membranes were developed by phase inversion technique and used in homotypic and organotypic culture systems. The morphological and functional characteristics of cells in the organotypic co-culture membrane systems were evaluated in comparison with homotypic cultures and traditional systems. Hepatocytes in the organotypic co-culture systems exhibit compact polyhedral cells with round nuclei and well demarcated cellecell borders like in vivo, as a result of heterotypic interaction with HUVECs. In addition HUVECs formed tube-like structures directly through the interactions with the membranes and hepatocytes and indirectly through the secretion of ECM proteins which secretion improved in the organotypic co-culture membrane systems. The heterotypic cellecell contacts have beneficial effect on the hepatocyte albumin production, urea synthesis and drug biotransformation. The developed organotypic co-culture membrane systems elicit liver specific functions in vitro and could be applied for the realization of engineered liver tissues to be used in tissue engineering, drug metabolism studies and bioartificial liver devices. (literal)

Prodotto di