New developments for the controlled fabrication of microstructured multiphase bioreactor using membrane emulsification technology (Articolo in rivista)

Type
Label
  • New developments for the controlled fabrication of microstructured multiphase bioreactor using membrane emulsification technology (Articolo in rivista) (literal)
Anno
  • 2009-01-01T00:00:00+01:00 (literal)
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#doi
  • 10.1016/j.nbt.2009.06.533  (literal)
Alternative label
  • Piacentini, E.; Giorno, L.; Mazzei, R.; Drioli, E. (2009)
    New developments for the controlled fabrication of microstructured multiphase bioreactor using membrane emulsification technology
    in New biotechnology (Print)
    (literal)
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#autori
  • Piacentini, E.; Giorno, L.; Mazzei, R.; Drioli, E. (literal)
Pagina inizio
  • S168 (literal)
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#numeroVolume
  • 25 (literal)
Rivista
Note
  • ISI Web of Science (WOS) (literal)
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#affiliazioni
  • ITM-CNR (literal)
Titolo
  • New developments for the controlled fabrication of microstructured multiphase bioreactor using membrane emulsification technology (literal)
Abstract
  • Over the past 20 years, there has been a growing interest in 'membrane emulsification' (ME). The distinguishing feature is that the resulting droplet size is controlled by the choice of the microporous membrane and not by drop breakup using shear or impact stress generated by mechanical agitation. The technique is highly attractive given its potentially lower energy demands and greater control over drop size distribution. Benefits of membrane emulsification for phase transfer biocatalysis could derive from low shear properties and structured phase with fine droplets. In this work, oil-in-water (O/W) emulsions with an heterogenized enzyme was produced by ME. The lipase was used as model enzyme. Lipase is an enantioselective phase transfer biocatalyst frequently used in esterifications, transesterifications and hydrolysis reactions accepting a broad range of even hydrophobic substrates with vast industrial importance. The enantioselective hydrolysis of racemic naproxen methyl ester was used a reaction model to produce (S)-naproxen, a member of the arylacetic acid group of nonsteroidal anti-inflammatory drugs. When emulsion is prepared with ME, lipase is distributed at the O/W interface while drops grow at the membrane pore opening, whereas the substrate is dissolved in the dispersed phase. Both droplets micromanufacturing and biocatalysts immobilization can be performed simultaneously and continuously. The interface with controlled and uniform size provided a constant reaction interface at steady-state. This methodology permits a controlled fabrication of monodispersed microstructured biocatalytic emulsion interface for a highly efficient enzymatic reaction. This new precisely controlled methodology to fabricate microstructured multiphase bioreactor was compared with conventional methods. Low shear stress and enzyme optimal spatial arrangement at the stable and constant oil/water interface permitted to obtain very high enantioselectivity (100%) at high conversion degree (up to 90% of the (S)-ester). In addition, thanks to the highly droplet uniformity and stability the methodology offered a possibility to accurately evaluate catalyst basic parameters such as the hydrodynamic diameter. This information is useful in the bioreactor optimization design. In this work, macromolecule diameter at the interface was evaluated and compared to the molecular diameter calculated from crystallographic data. The ME technology offers a novel, easy and scalable technique for producing microfabricated reactor for a large variety of process implementation in biotechnology, biomedicine, food, waste water treatment. The flexibility of the method permits to produce a wide variety of functional microparticles having targeted properties in terms of size distribution and composition by mixing various chemical or biological material and controlling membrane process parameters. (literal)
Prodotto di
Autore CNR

Incoming links:


Prodotto
Autore CNR di
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#rivistaDi
data.CNR.it