http://www.cnr.it/ontology/cnr/individuo/prodotto/ID55082

Human hepatocyte functions in a crossed hollow fiber membrane bioreactor (Articolo in rivista)

Type

Prodotto della ricerca (Classe)
Articolo in rivista (Classe)

Label

Human hepatocyte functions in a crossed hollow fiber membrane bioreactor (Articolo in rivista) (literal)

Anno

2009-01-01T00:00:00+01:00 (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#doi

10.1016/j.biomaterials.2009.01.011 (literal)

Alternative label

Loredana De Bartolo, Simona Salerno, Efrem Curcio, Antonella Piscioneri, Maria Rende, Sabrina Morelli, Franco Tasselli, Augustinus Bader, Enrico Drioli (2009)
Human hepatocyte functions in a crossed hollow fiber membrane bioreactor
in Biomaterials
(literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#autori

Loredana De Bartolo, Simona Salerno, Efrem Curcio, Antonella Piscioneri, Maria Rende, Sabrina Morelli, Franco Tasselli, Augustinus Bader, Enrico Drioli (literal)

Pagina inizio

2531 (literal)

Pagina fine

2543 (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#numeroVolume

30 (literal)

Rivista

Biomaterials (Rivista)

Note

ISI Web of Science (WOS) (literal)
Scopu (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#affiliazioni

Department of Chemical Engineering and Materials, University of Calabria, Rende (CS) CNR Institute on Membrane Technology- Rende (CS) Biomedical-Biotechnological Center, BBZ, University of Leipzig, Germany (literal)

Titolo

Human hepatocyte functions in a crossed hollow fiber membrane bioreactor (literal)

Abstract

An important challenge in liver tissue engineering is the development of bioartificial systems that are able to favour the liver reconstruction and to modulate liver cell behaviour. A crossed hollow fiber membrane bioreactor was developed to support the long-term maintenance and differentiation of human hepatocytes. The bioreactor consists of two types of hollow fiber (HF) membranes with different molecular weight cut-off (MWCO) and physico-chemical properties crossassembled in alternating manner: modified polyetheretherketone (PEEK-WC) and polyethersulfone (PES), used for the medium inflow and outflow, respectively. The combination of these two fiber set produces an extracapillary network for the adhesion of cells and a high mass exchange through the cross-flow of culture medium. The transport of liver specific products such as albumin and urea together with the transport of drug such as diazepam was modelled and compared with the experimental metabolic data. The theoretical metabolite concentration differed 7.5% for albumin and 5% for urea with respect to experimental data. The optimised perfusion conditions of the bioreactor allowed the maintenance of liver functions in terms of urea synthesis, albumin secretion and diazepam biotransformation up to 18 days of culture. In particular the good performance of the bioreactor was confirmed by the high rate of urea synthesis (28.7 mg/h 106 cells) and diazepam biotransformation. In the bioreactor human hepatocytes expressed at high levels the individual cytochrome P450 isoenzymes involved in the diazepam metabolism. The results demonstrated that crossed HF membrane bioreactor is able to support the maintenance of primary human hepatocytes preserving their liver specific functions for all investigated period. This device may be a potential tool in the liver tissue engineering for drug metabolism/toxicity testing and study of disease pathogenesis alternatively to animal experimentation. (literal)

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