Label-free cancer markers detection by capacitance biochip (Articolo in rivista)

Type

• Articolo in rivista (Classe)
• Prodotto della ricerca (Classe)

Label

• Label-free cancer markers detection by capacitance biochip (Articolo in rivista) (literal)

Anno

• 2009-01-01T00:00:00+01:00 (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#doi

• 10.1016/j.snb.2008.09.050 (literal)

Alternative label

• Carrara Sandro (1), Bhalla Viiayender (1), Stagni Claudio (1), Benini Luca (2), Ferretti Anna (1), Valle Francesco (1), Gallotta Andrea (3), Ricco Bruno (2), Samori Bruno (1) (2009)
  Label-free cancer markers detection by capacitance biochip
  in Sensors and actuators. B, Chemical (Print)
  (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#autori

• Carrara Sandro (1), Bhalla Viiayender (1), Stagni Claudio (1), Benini Luca (2), Ferretti Anna (1), Valle Francesco (1), Gallotta Andrea (3), Ricco Bruno (2), Samori Bruno (1) (literal)

Pagina inizio

• 163 (literal)

Pagina fine

• 172 (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#numeroVolume

• 136 (literal)

Rivista

• Sensors and actuators. B, Chemical (Rivista)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#numeroFascicolo

• 1 (literal)

Note

• ISI Web of Science (WOS) (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#affiliazioni

• (1) Biochemistry Department, University of Bologna, Via Irnerio, 48 Bologna, Italy (2) DEIS Department, University of Bologna, Viale Risorgimento 2, Bologna, Italy (3) Xeptagen S.p.A, Via delle Industrie 9, Venezia Mestre, Italy (literal)

Titolo

• Label-free cancer markers detection by capacitance biochip (literal)

Abstract

• This paper investigates technological advancements in developing reliable technologies for labelfreedetection of cancermarkers. Capacitancedetection was used successfully to develop an immunosensor. Rigorous probe immobilization was implemented to obtain reliable capacitance measurements. The commonly considered alkanethiols for proteins immobilization do not allow sufficiently stable capacitance measurements. Therefore, new ethylene-glycol functionalized alkanethiols were used as more suitable materials to develop lab-on-a-chip detection of cancermarkers. Results from AFM, Chemi-luminescence, Fluorescence, Cyclic Voltammetry, QCM, and capacitance measurements showed that three (ethylene-glycol) alkanethiols highly stabilized the sensing surfaces in time. The measured capacitance on the antibody layers was in the range of 60-70 nF/cm2 and 80-115 nF/cm2 for the antigen detection. The developed system was utilized to detect the Hepatocarcinoma marker SCCA, and the whole IgM fraction from Hepatocarcinoma patient's serum. Electrode by electrode reproducibility within the same chip increased by one order of magnitude (variations were reduced from 30-40% down to less than 2%) using these new alkanethiols, while the reached limit in sensitivity was close to 2.43 ?g/ml (literal)

Prodotto di

• Nanotecnologie di materiali multifunzionali e sistemi biologici (PM.P05.010.001) (Modulo)
• Istituto per lo studio dei materiali nanostrutturati (ISMN) (Istituto)

Autore CNR

• FRANCESCO VALLE (Unità di personale interno)

Incoming links:

Prodotto

• Istituto per lo studio dei materiali nanostrutturati (ISMN) (Istituto)
• Nanotecnologie di materiali multifunzionali e sistemi biologici (PM.P05.010.001) (Modulo)

Autore CNR di

• FRANCESCO VALLE (Unità di personale interno)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#rivistaDi

• Sensors and actuators. B, Chemical (Rivista)