http://www.cnr.it/ontology/cnr/individuo/prodotto/ID53097
Fate of Prions in Soil : Interactions of RecPrP with Organic Matter of Soil Aggregates as revealed by LTA-PAS (Articolo in rivista)
- Type
- Label
- Fate of Prions in Soil : Interactions of RecPrP with Organic Matter of Soil Aggregates as revealed by LTA-PAS (Articolo in rivista) (literal)
- Anno
- 2008-01-01T00:00:00+01:00 (literal)
- Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#doi
- 10.1021/es071314q (literal)
- Alternative label
- Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#autori
- Amaranta Pucci; Luigi Paolo D'Acqui; Luca Calamai (literal)
- Pagina inizio
- Pagina fine
- Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#numeroVolume
- Rivista
- Note
- ISI Web of Science (WOS) (literal)
- Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#affiliazioni
- Dipartimento di Scienza del Suolo e Nutrizione della Pianta, Università degli Studi di Firenze, Piazzale delle Cascine 28, 50144 Firenze, Italy;
Istituto per lo Studio degli Ecosistemi, CNR Via Madonna del Piano 10, 50019 Sesto Fiorentino (FI), Italy (literal)
- Titolo
- Fate of Prions in Soil : Interactions of RecPrP with Organic Matter of Soil Aggregates as revealed by LTA-PAS (literal)
- Abstract
- The contribution of soil organic matter (OM) to the adsorption of a recombinant prion protein (RecPrP) was studied in microcosm systems (soil aggregates from two different soils) before and after OM removal by low temperature ashing (LTA). The LTA technique allows a controlled removal of OM layer by layer, like a peeling of an onion skin, with minimal disturbance of the mineral matrix. Soil aggregates were selected as a representative model of the \"in situ\" conditions. Adsorption from batch vs percolation experiments were compared, and the aggregates were characterized by photoacustic Fourier- transform IR spectroscopy (PAS-FTIR). High affinity (H-type) adsorption isotherms were found with complete removal of RecPrP from solution for protein/soil ratios up to 1:62.5. OM removal from aggregates decreased the adsorbed RecPrP in amounts corresponding to 330-1000 ?g mg-1 of soil organic carbon (OC) indicating that native OM has specific adsorption capacity comparable and/or superior to the mineral matrix. The coupled LTA-PAS-FTIR approach demonstrated that, albeit OM composition was homogeneous throughout the aggregates, its presence in the most external surfaces of the aggregates affects the diffusion dynamics of RecPrP within the aggregates during percolation. (literal)
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