http://www.cnr.it/ontology/cnr/individuo/prodotto/ID5309

Biofilm formation by Stenotrophomonas maltophilia: modulation by quinolones, trimethoprim-sulfamethoxazole, and ceftazidime (Articolo in rivista)

Type

Prodotto della ricerca (Classe)
Articolo in rivista (Classe)

Label

Biofilm formation by Stenotrophomonas maltophilia: modulation by quinolones, trimethoprim-sulfamethoxazole, and ceftazidime (Articolo in rivista) (literal)

Anno

2004-01-01T00:00:00+01:00 (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#doi

10.1128/AAC.48.1.151-160.2004 (literal)

Alternative label

G. DI BONAVENTURA; I. SPEDICATO; D. D'ANTONIO; I. ROBUFFO; R. PICCOLOMINI (2004)
Biofilm formation by Stenotrophomonas maltophilia: modulation by quinolones, trimethoprim-sulfamethoxazole, and ceftazidime
in Antimicrobial agents and chemotherapy (Print); ASM, American society for microbiology, Washington, DC (Stati Uniti d'America)
(literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#autori

G. DI BONAVENTURA; I. SPEDICATO; D. D'ANTONIO; I. ROBUFFO; R. PICCOLOMINI (literal)

Pagina inizio

151 (literal)

Pagina fine

160 (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#url

http://aac.asm.org/content/48/1/151 (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#numeroVolume

48 (literal)

Rivista

Antimicrobial agents and chemotherapy (Rivista)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#pagineTotali

10 (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#numeroFascicolo

1 (literal)

Note

ISI Web of Science (WOS) (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#affiliazioni

Univ G DAnnunzio, Ctr Sci Invecchiamento, Chieti, Italy Univ G DAnnunzio, Dipartimento Sci Biomed, Lab Microbiol Clin, I-66100 Chieti, Italy Univ G DAnnunzio, Ctr Sci Invecchiamento, Chieti, Italy CNR, Ist Trapianti Organo & Immunocitol, Sez Chieti, Italy ASL, Serv Microbiol Clin, Dipartimento Ematol & Oncol, Osped Spirito Santo, Pescara, Italy (literal)

Titolo

Biofilm formation by Stenotrophomonas maltophilia: modulation by quinolones, trimethoprim-sulfamethoxazole, and ceftazidime (literal)

Abstract

We investigated the in vitro effects of seven fluoroquinolones (ciprofloxacin, grepafloxacin, levofloxacin, moxifloxacin, norfloxacin, ofloxacin, and rufloxacin), compared to those of trimethoprim-sulfamethoxazole (SXT) and ceftazidime on total biomass and cell viability of Stenotrophomonas maltophilia biofilm. S. maltophilia attached rapidly to polystyrene, within 2 h of incubation, and then biofilm formation increased over time, reaching maximum growth at 24 h. In the presence of fluoroquinolones at one-half and one-fourth the MIC, biofilm biomass was significantly (P < 0.01) reduced to 55 to 70% and 66 to 76% of original mass, respectively. Ceftazidime and SXT did not exert any activity. Biofilm bacterial viability was significantly reduced by all antibiotics tested at one-half the MIC. At one-fourth the MIC all antibiotics, except levofloxacin, significantly reduced viability. Treatment of preformed biofilms with bactericidal concentrations (500, 100, and 50 mu g/ml) of all fluoroquinolones caused, except for norfloxacin, significant reduction of biofilm biomass to 29.5 to 78.8, 64.1 to 83.6, and 70.5 to 82.8% of original mass, respectively. SXT exerted significant activity at 500 mu g/ml only. Ceftazidime was completely inactive. Rufloxacin exhibited the highest activity on preformed biofilm viability, significantly decreasing viable counts by 0.6, 5.4, and 17.1% at 500, 100, and 50 mu g/ml, respectively. Our results show that (i) subinhibitory (one-half and one-fourth the MIC) concentrations of fluoroquinolones inhibit adherence of S. maltophilia to polystyrene and (ii) clinically achievable concentrations (50 and 100 mu g/ml) of rufloxacin are able to eradicate preformed S. maltophilia biofilm. (literal)

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