http://www.cnr.it/ontology/cnr/individuo/prodotto/ID5247
Cellular and molecular events during chondrogenesis of human mesenchymal stromal cells grown in a three-dimensional hyaluronan based scaffold (Articolo in rivista)
- Type
- Label
- Cellular and molecular events during chondrogenesis of human mesenchymal stromal cells grown in a three-dimensional hyaluronan based scaffold (Articolo in rivista) (literal)
- Anno
- 2005-01-01T00:00:00+01:00 (literal)
- Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#doi
- 10.1016/j.biomaterials.2005.02.031 (literal)
- Alternative label
- Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#autori
- Gina Lisignoli; Sandra Cristino; Anna Piacentini; Stefania Toneguzzi; Francesco Grassi; Carola Cavallo; Nicoletta Zini; Liliana Solimando; Nadir Mario Maraldi; Andrea Facchini (literal)
- Pagina inizio
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- Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#numeroVolume
- Rivista
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- ISI Web of Science (WOS) (literal)
- Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#affiliazioni
- Laboratorio di Immunologia e Genetica, Istituti Ortopedici Rizzoli, Via di Barbiano 1/10, 40136 Bologna, Italy; ITOI-CNR, Sezione di Bologna c/o Istituti Ortopedici Rizzoli, 40136 Bologna, Italy; Laboratorio di Biologia Cellulare e Microscopia Elettronica, Istituti Ortopedici Rizzoli, 40136 Bologna, Italy; Dipartimento di Medicina Interna e Gastroenterologia, Università degli Studi di Bologna, 40138 Bologna, Italy (literal)
- Titolo
- Cellular and molecular events during chondrogenesis of human mesenchymal stromal cells grown in a three-dimensional hyaluronan based scaffold (literal)
- Abstract
- Mesenchymal stromal cells (MSCs) seem to be a good alternative to chondrocytes for cartilage regeneration. To obtain new information on the sequence of cellular and molecular events during in vitro chondrogenic differentiation we analysed MSCs on a widely used hyaluronic acid biomaterial (Hyaff(g) -11). Cellular differentiation was induced using two different concentrations of TGF beta 1 (10 and 20 ng/ml) and the process was analysed at different time points (24 h, and 7, 14 21 and 28 days) using techniques of light and electron microscopy, real-time PCR and immunohistochemistry. We found that without TGF beta MSCs did not survive while in the presence of TGF beta the cells significantly proliferated from day 7 until day 28. Light and electron microscopy showed that TGF beta at 20 ng/ml better induced the formation of cartilage-like tissue. Real-time PCR showed an increased expression of collagen type II, IX and aggrecan associated to a down-regulation of collagen type I. Immunohistochemical analysis confirmed that collagen type I was down-modulated while collagen type II increased from day 14 to day 28.
These data clearly showed that higher concentrations of TGF beta (20 ng/ml) induce chondrogenesis of MSCs on Hyaff(g)-11 scaffold better than 10 ng/ml of TGF beta. This process is characterized by a sequence of cellular and molecular events that deal with the in vitro formation of a cartilage-like tissue. (literal)
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