Effects of antisense mediated inhibition of cathepsin K on human osteoclasts obtained from peripheral blood (Articolo in rivista)

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  • Effects of antisense mediated inhibition of cathepsin K on human osteoclasts obtained from peripheral blood (Articolo in rivista) (literal)
Anno
  • 2006-01-01T00:00:00+01:00 (literal)
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#doi
  • 10.1002/jor.20209 (literal)
Alternative label
  • Sofia Avnet; Annavera Lamolinara; Nicoletta Zini; Liliana Solimando; Gianni Quacquaruccio; Donatella Granchi; Nadir Mario Maraldi; Armando Giunti; Nicola Baldini (2006)
    Effects of antisense mediated inhibition of cathepsin K on human osteoclasts obtained from peripheral blood
    in Journal of orthopaedic research
    (literal)
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#autori
  • Sofia Avnet; Annavera Lamolinara; Nicoletta Zini; Liliana Solimando; Gianni Quacquaruccio; Donatella Granchi; Nadir Mario Maraldi; Armando Giunti; Nicola Baldini (literal)
Pagina inizio
  • 1699 (literal)
Pagina fine
  • 1708 (literal)
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#numeroVolume
  • 24 (literal)
Rivista
Note
  • ISI Web of Science (WOS) (literal)
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#affiliazioni
  • Laboratory for Pathophysiology, Istituti Ortopedici Rizzoli, v. di barbiano 1/10, 40136 Bologna, Italy; Department of Human Anatomy and Pathophysiology of Musculoskeletal System, University of Bologna, Bologna, Italy; ITOI-CNR, Unit of Bologna, c/o Istituti Ortopedici Rizzoli, Bologna, Italy; Laboratory of Cell Biology and Electron Microscopy, Istituti Ortopedici Rizzoli, Bologna, Italy (literal)
Titolo
  • Effects of antisense mediated inhibition of cathepsin K on human osteoclasts obtained from peripheral blood (literal)
Abstract
  • Cathepsin K is a cystein protease that displays a proteolytic activity against Type I collagen and is abundantly and selectively expressed in osteoclasts where it plays a critical role in bone degradation. Its direct role in bone tissue has been defined by knock-out mice studies and inhibiting strategies in animals models. However, direct proof of cathepsin K function in human osteoclast model in vitro is lacking. The aim of this study is to analyze cathepsin K expression and localization in human osteoclasts obtained from peripheral blood and to examine cathepsin K function in these cells by antisense oligodeoxynucleotide (AS-ODN) strategy. AS-ODN was added to the culture of osteoclast precursors induced to differentiate by RANKL and M-CSF. AS-ODN treatment produced a significant down-regulation of cathepsin K mRNA (>80%) and protein expression, as verified respectively by Real-time PCR and by immunocytochemistry or Western blot. The cathepsin K inhibition caused an impairment of resorption activity as evaluated by a pit formation assay ( p=0.045) and by electron microscopy, while the acidification process was unaffected.We demonstrated that antisense strategies against cathepsin K are selectively effective to inhibit resorption activity in human osteoclasts, like in animal models. (literal)
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