http://www.cnr.it/ontology/cnr/individuo/prodotto/ID5217
Effects of antisense mediated inhibition of cathepsin K on human osteoclasts obtained from peripheral blood (Articolo in rivista)
- Type
- Label
- Effects of antisense mediated inhibition of cathepsin K on human osteoclasts obtained from peripheral blood (Articolo in rivista) (literal)
- Anno
- 2006-01-01T00:00:00+01:00 (literal)
- Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#doi
- 10.1002/jor.20209 (literal)
- Alternative label
- Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#autori
- Sofia Avnet; Annavera Lamolinara; Nicoletta Zini; Liliana Solimando; Gianni Quacquaruccio; Donatella Granchi; Nadir Mario Maraldi; Armando Giunti; Nicola Baldini (literal)
- Pagina inizio
- Pagina fine
- Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#numeroVolume
- Rivista
- Note
- ISI Web of Science (WOS) (literal)
- Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#affiliazioni
- Laboratory for Pathophysiology, Istituti Ortopedici Rizzoli, v. di barbiano 1/10, 40136 Bologna, Italy;
Department of Human Anatomy and Pathophysiology of Musculoskeletal System, University of Bologna, Bologna, Italy;
ITOI-CNR, Unit of Bologna, c/o Istituti Ortopedici Rizzoli, Bologna, Italy;
Laboratory of Cell Biology and Electron Microscopy, Istituti Ortopedici Rizzoli, Bologna, Italy (literal)
- Titolo
- Effects of antisense mediated inhibition of cathepsin K on human osteoclasts obtained from peripheral blood (literal)
- Abstract
- Cathepsin K is a cystein protease that displays a proteolytic activity against Type I
collagen and is abundantly and selectively expressed in osteoclasts where it plays a critical role in
bone degradation. Its direct role in bone tissue has been defined by knock-out mice studies and
inhibiting strategies in animals models. However, direct proof of cathepsin K function in human
osteoclast model in vitro is lacking. The aim of this study is to analyze cathepsin K expression and
localization in human osteoclasts obtained from peripheral blood and to examine cathepsin K
function in these cells by antisense oligodeoxynucleotide (AS-ODN) strategy. AS-ODN was added to
the culture of osteoclast precursors induced to differentiate by RANKL and M-CSF. AS-ODN
treatment produced a significant down-regulation of cathepsin K mRNA (>80%) and protein
expression, as verified respectively by Real-time PCR and by immunocytochemistry or Western blot.
The cathepsin K inhibition caused an impairment of resorption activity as evaluated by a pit
formation assay ( p=0.045) and by electron microscopy, while the acidification process was
unaffected.We demonstrated that antisense strategies against cathepsin K are selectively effective
to inhibit resorption activity in human osteoclasts, like in animal models. (literal)
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