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Transient maintenance in bioreactor improves health of neuronal cells. (Articolo in rivista)

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Transient maintenance in bioreactor improves health of neuronal cells. (Articolo in rivista) (literal)

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Di Loreto S1; Sebastiani P1; Benedetti E2; Zimmitti V2; Caracciolo V2; Amicarelli F2; Cimini AM2; Adorno D1. (2006)
Transient maintenance in bioreactor improves health of neuronal cells.
in In vitro cellular & developmental biology. Animal (Print); Tissue Culture Association, Columbia (Stati Uniti d'America)
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Di Loreto S1; Sebastiani P1; Benedetti E2; Zimmitti V2; Caracciolo V2; Amicarelli F2; Cimini AM2; Adorno D1. (literal)

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1 Institute of Organ Transplants and Immunocytology, Consiglio Nazionale delle Ricerche, L'Aquila, Italy; 2 Department of Basic and Applied Biology, University of L'Aquila, L'Aquila, Italy (literal)

Titolo

Transient maintenance in bioreactor improves health of neuronal cells. (literal)

Abstract

To examine whether a neuronal cell suspension can be held in vitro for a relatively short period without compromising survival rates and functionality, we have set up an experimental protocol planning 24 h of suspension culture in a rotary wall vessel (RWV) bioreactor before plating in a conventional adherent system. Apoptosis measurement and activated caspase-8, -9, and -3 detection have demonstrated that survey of the cells was not affected. The activity of major antioxidant enzymes (AOE), superoxide dismutase (SOD), glutathione peroxidase (GSH-Px), and catalase (CAT), was significantly decreased in RWV-maintained cells. A significant decrease of tumor necrosis factor-? (TNF-?) and interleukin- 1? (IL-1?) is coupled with a level of activated nuclear factor-?B (NF-?B) protein significantly lower in RVW cells than in the control. On the contrary, the level of IL-6 expression did not change between the test and the control. A significant up-regulation of growth-associated protein-43 (GAP-43), peroxisome proliferator-activated receptor-?/? (PPAR?/?), and acyl-CoA synthetase 2 (ACS2) in RWV cells has been detected. We provide the evidence that primary neuronal cells, at an early stage of development, can be maintained in a suspension condition before adherent plating. This experimental environment does not induce detrimental effects but may have an activator role, leading cells to development and maturation in a tridimensional state. (literal)

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