http://www.cnr.it/ontology/cnr/individuo/prodotto/ID51853
Large-scale in vitro propagation of giant reed (Arundo donax L.), a promising biomass species (Articolo in rivista)
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- Large-scale in vitro propagation of giant reed (Arundo donax L.), a promising biomass species (Articolo in rivista) (literal)
- Anno
- 2011-01-01T00:00:00+01:00 (literal)
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- Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#autori
- Cavallaro V.; Tringali S.; Patanè C.; (literal)
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- Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#numeroVolume
- Rivista
- Note
- ISI Web of Science (WOS) (literal)
- Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#affiliazioni
- Titolo
- Large-scale in vitro propagation of giant reed (Arundo donax L.), a promising biomass species (literal)
- Abstract
- Giant reed (Arundo donax L.), a promising energy crop, is vegetatively-propagated from fragments of stems and
rhizomes. This may limit large-scale cultivation, since it is time-consuming and involves considerable cost and effort.
Tissue culture is an alternative to conventional methods of vegetative propagation and may represent a useful tool for
large-scale propagation of plants for biomass production programmes. This report describes a protocol for the largescale
in vitro propagation of giant reed by adventitious bud formation. Stem nodes with dormant buds proved to be
the most effective to initiate in vitro cultures giving the highest percentage of differentiated shoots (77%) compared
to the other plant fractions tested. A sterilisation procedure using 5 g l-1 HgCl2 enabled the production of sterile
explants. Moreover, early results indicated that late Autumn excision dates not only gave a higher percentage of welldeveloped
shoots ( > 80%), but also a lower level of bacterial contamination (15 - 20%). 6-Benzylaminopurine (BAP)
at 3.0 mg l-1 was most effective in promoting shoot multiplication when added to a basal medium containing
Murashige and Skoog (MS) macro- and micro-nutrients, Morel's vitamins, 30 g l-1 sucrose, and 7.0 g l-1 bacteriological
agar supplemented with 1.0 mg l-1 indole-3-acetic acid (IAA) and 0.05 mg l-1 gibberellic acid (GA3). Rooting was
successfully induced on the same basal medium used for proliferation, modified by halving the MS macro-nutrients
and replacing BAP and the other growth regulators with 2.0 mg l-1 1-naphthaleneacetic acid (NAA). Successful
acclimatisation (> 95% survival) of plantlets was carried out, even in late Winter, in a cold greenhouse or under
simpler facilities such as shade nets. (literal)
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