http://www.cnr.it/ontology/cnr/individuo/prodotto/ID5175
Relevance of post-transplant HLA class I and classII antibodies on renal graft outcome (Articolo in rivista)
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- Relevance of post-transplant HLA class I and classII antibodies on renal graft outcome (Articolo in rivista) (literal)
- Anno
- 2001-01-01T00:00:00+01:00 (literal)
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Piazza A., Poggi E., Borrelli L., Valeri M., Buonomo O., Servetti S., Casciani C.U., Adorno D. (2001)
Relevance of post-transplant HLA class I and classII antibodies on renal graft outcome
in Transplantation proceedings
(literal)
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- Piazza A., Poggi E., Borrelli L., Valeri M., Buonomo O., Servetti S., Casciani C.U., Adorno D. (literal)
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- This study was designed to characterise HLA class I and II specificity of donor-specific antibodies (DS-Abs) and to highlight the relevance of HLA specificity on renal graft outcome.We monitored DS-Abs production in 149 kidney transplanted patients (pts) using flow cytometry crossmatch (FCXM) and FlowPRA beads assay. Analysing graft outcome, we found a higher incidence of both acute rejection and graft failure (GF) in FCXM-positive pts. than in the negative ones (ARj: 71% vs. 17.8%, p<0.00001; GF: 29% vs. 1.7%, p<0.00001). HLA specificity determination showed the production of class I and II Abs in 40% of IgG FCXM-positive pts, only class II Abs in 20% and class I alone in the remaining 40%. Correlating GF and HLA DS-Abs specificity we found that 43% of graft losses occurred in only class II DS-Abs positive pts while the remaining 57% occurred in pts showing class I only or both class I and II Abs. In conclusion our data suggests that post-transplant flow cytometric monitoring of DS-Abs allows to estimate the HLA-specific immune response in kidney transplanted pts. and emphasises the negative influence not only of HLA class I but also of HLA class II donor-specific sensitisation on renal graft survival. (literal)
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- Titolo
- Relevance of post-transplant HLA class I and classII antibodies on renal graft outcome (literal)
- Abstract
- Development of HLA antibodies (Abs) specific for graft mismatched antigens is the most important factor for rejection occurrence and poor graft survival. This study was designed to characterise HLA class I and II specificity of donor-specific antibodies (DS-Abs) and to highlight the relevance of HLA specificity on renal graft outcome. We monitored DS-Abs production in 149 kidney transplanted patients (pts) for 1 year after transplantation using flow cytometry crossmatch (FCXM) and FlowPRA beads assay. Analysing graft outcome (2 years follow up), we found a higher incidence of both acute rejection and graft failure (GF) in FCXM-positive pts. than in the negative ones (ARj: 71% vs. 17.8%, p<0.00001; GF: 29% vs. 1.7%, p<0.00001). HLA specificity determination showed the production of class I and II Abs in 40% of IgG FCXM-positive pts, only class II Abs in 20% and class I alone in the remaining 40%. The analysis of class I DS-Abs specificity highlighted that 2 pts produced Abs against specificity included in recipients HLA CREG; one of them lost the graft during our follow up period. In the remaining pts, the Abs presented extra-CREG (9 cases) or single allelic (5 cases) specificity. As regard class II specificity only 1 patient produced Abs directed against a public antigens of the graft. Six subjects presented Abs specific for mismatched DR and/or DQ donors antigens and in 5 cases no specificity was defined. Correlating GF and HLA DS-Abs specificity we found that 43% of graft losses occurred in only class II DS-Abs positive pts while the remaining 57% occurred in pts showing class I only or both class I and II Abs. In conclusion our data suggests that post-transplant flow cytometric monitoring of DS-Abs allows to estimate the HLA-specific immune response in kidney transplanted pts. and emphasises the negative influence not only of HLA class I but also of HLA class II donor-specific sensitisation on renal graft survival. (literal)
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