Relevance of post-transplant HLA class I and classII antibodies on renal graft outcome (Articolo in rivista)

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Label
  • Relevance of post-transplant HLA class I and classII antibodies on renal graft outcome (Articolo in rivista) (literal)
Anno
  • 2001-01-01T00:00:00+01:00 (literal)
Alternative label
  • Piazza A., Poggi E., Borrelli L., Valeri M., Buonomo O., Servetti S., Casciani C.U., Adorno D. (2001)
    Relevance of post-transplant HLA class I and classII antibodies on renal graft outcome
    in Transplantation proceedings
    (literal)
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  • Piazza A., Poggi E., Borrelli L., Valeri M., Buonomo O., Servetti S., Casciani C.U., Adorno D. (literal)
Pagina inizio
  • 478 (literal)
Pagina fine
  • 480 (literal)
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  • 33 (literal)
Rivista
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  • This study was designed to characterise HLA class I and II specificity of donor-specific antibodies (DS-Abs) and to highlight the relevance of HLA specificity on renal graft outcome.We monitored DS-Abs production in 149 kidney transplanted patients (pts) using flow cytometry crossmatch (FCXM) and FlowPRA beads assay. Analysing graft outcome, we found a higher incidence of both acute rejection and graft failure (GF) in FCXM-positive pts. than in the negative ones (ARj: 71% vs. 17.8%, p<0.00001; GF: 29% vs. 1.7%, p<0.00001). HLA specificity determination showed the production of class I and II Abs in 40% of IgG FCXM-positive pts, only class II Abs in 20% and class I alone in the remaining 40%. Correlating GF and HLA DS-Abs specificity we found that 43% of graft losses occurred in only class II DS-Abs positive pts while the remaining 57% occurred in pts showing class I only or both class I and II Abs. In conclusion our data suggests that post-transplant flow cytometric monitoring of DS-Abs allows to estimate the HLA-specific immune response in kidney transplanted pts. and emphasises the negative influence not only of HLA class I but also of HLA class II donor-specific sensitisation on renal graft survival. (literal)
Note
  • ISI Web of Science (WOS) (literal)
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#affiliazioni
  • ITOI CNR - Sezione di Roma; Università degli Studi di Roma \"Tor Vergata\" (literal)
Titolo
  • Relevance of post-transplant HLA class I and classII antibodies on renal graft outcome (literal)
Abstract
  • Development of HLA antibodies (Abs) specific for graft mismatched antigens is the most important factor for rejection occurrence and poor graft survival. This study was designed to characterise HLA class I and II specificity of donor-specific antibodies (DS-Abs) and to highlight the relevance of HLA specificity on renal graft outcome. We monitored DS-Abs production in 149 kidney transplanted patients (pts) for 1 year after transplantation using flow cytometry crossmatch (FCXM) and FlowPRA beads assay. Analysing graft outcome (2 years follow up), we found a higher incidence of both acute rejection and graft failure (GF) in FCXM-positive pts. than in the negative ones (ARj: 71% vs. 17.8%, p<0.00001; GF: 29% vs. 1.7%, p<0.00001). HLA specificity determination showed the production of class I and II Abs in 40% of IgG FCXM-positive pts, only class II Abs in 20% and class I alone in the remaining 40%. The analysis of class I DS-Abs specificity highlighted that 2 pts produced Abs against specificity included in recipients’ HLA CREG; one of them lost the graft during our follow up period. In the remaining pts, the Abs presented extra-CREG (9 cases) or single allelic (5 cases) specificity. As regard class II specificity only 1 patient produced Abs directed against a public antigens of the graft. Six subjects presented Abs specific for mismatched DR and/or DQ donor’s antigens and in 5 cases no specificity was defined. Correlating GF and HLA DS-Abs specificity we found that 43% of graft losses occurred in only class II DS-Abs positive pts while the remaining 57% occurred in pts showing class I only or both class I and II Abs. In conclusion our data suggests that post-transplant flow cytometric monitoring of DS-Abs allows to estimate the HLA-specific immune response in kidney transplanted pts. and emphasises the negative influence not only of HLA class I but also of HLA class II donor-specific sensitisation on renal graft survival. (literal)
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