Improving screening strategies for HLA-specific antibody detection in renal transplant recipients (Articolo in rivista)

Type
Label
  • Improving screening strategies for HLA-specific antibody detection in renal transplant recipients (Articolo in rivista) (literal)
Anno
  • 2001-01-01T00:00:00+01:00 (literal)
Alternative label
  • Piazza A., Poggi E., Borrelli L., Monaco P.I., Ozzella G., Cortini C., Torlone N., Servetti S., Casciani C.U., Adorno D. (2001)
    Improving screening strategies for HLA-specific antibody detection in renal transplant recipients
    in Transplantation proceedings
    (literal)
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  • Piazza A., Poggi E., Borrelli L., Monaco P.I., Ozzella G., Cortini C., Torlone N., Servetti S., Casciani C.U., Adorno D. (literal)
Pagina inizio
  • 478 (literal)
Pagina fine
  • 480 (literal)
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  • 33 (literal)
Rivista
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  • In order to improve both aspects in revealing a pre-sensitisation status, 297 sera from 221 patients awaiting renal transplantation were screened using Amos-CDCPRA and FlowPRA class I and II beads techniques. Overall concordance was 72.7%. Moreover 48 (16.2%) serum samples showed “false negative” CDCPRA results and 33 (11.1%) sera presented “false positive” CDCPRA results. We highlighted higher mean PRA levels using FlowPRA than CDCPRA technique (66%±26% vs. 37%±25%, p<0.0001). To evidence the weight of different immunising events on alloantibody production we analysed PRA results in patients receiving only one type of sensitisation (transfusion, previous transplant or pregnancy). A low incidence (4.1%)was found in the group of transfused patients (Ts-group), while more “false negative” CDCPRA results appeared in both the re-transplants group (Tx-group 18.2%, p=0.06) and in previously pregnant female patients (Pg-group 21,9%, p=0.01). FlowPRA data evidenced more sensitised subjects in the Tx-group (50%, p=0.0004) and in the Pg-group (34.1%, p=0.00865) than in Ts-group of patients (10.2%). We furthermore highlighted a high HLA class II positivity not only in Tx-group (55.2±33.6%) but also in Pg-group (49.8±32.6%); on the contrary it was very low in the Ts-group. FlowPRA seems to be an adequate tool for HLA-specific antibody screening in candidate renal transplant recipients able to identify patients with a high risk of poor graft outcome. (literal)
Note
  • ISI Web of Science (WOS) (literal)
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#affiliazioni
  • ITOI CNR- Sezione di Roma; Università degli Studi di Roma \"Tor Vergata\" (literal)
Titolo
  • Improving screening strategies for HLA-specific antibody detection in renal transplant recipients (literal)
Abstract
  • Sensitivity in detecting low levels of alloantibodies and specificity in revealing the presence of HLA-specific antibodies are the most important clinical features of a PRA screening technique. In order to improve both aspects in revealing a pre-sensitisation status, 297 sera from 221 patients awaiting renal transplantation were screened using Amos-CDCPRA and FlowPRA class I and II beads techniques. Overall concordance was 72.7%. Moreover 48 (16.2%) serum samples showed “false negative” CDCPRA results and 33 (11.1%) sera presented “false positive” CDCPRA results. As regards the percentage of PRA positivity, we highlighted higher mean PRA levels using FlowPRA than CDCPRA technique (66%±26% vs. 37%±25%, p<0.0001). To evidence the weight of different immunising events on alloantibody production we analysed PRA results in patients receiving only one type of sensitisation (transfusion, previous transplant or pregnancy). A low incidence (4.1%) of “false negative” CDCPRA results was found in the group of transfused patients (Ts-group), while more “false negative” CDCPRA results appeared in both the re-transplants group (Tx-group 18.2%, p=0.06) and in previously pregnant female patients (Pg-group 21,9%, p=0.01). Therefore, it may not be possible to classify a patient as non sensitised solely on CDCPRA assay basis. FlowPRA data evidenced more sensitised subjects in the Tx-group (50%, p=0.0004) and in the Pg-group (34.1%, p=0.00865) than in Ts-group of patients (10.2%). We furthermore highlighted a high HLA class II positivity not only in Tx-group (55.2±33.6%) but also in Pg-group (49.8±32.6%); on the contrary it was very low in the Ts-group. From these data, FlowPRA seems to be an adequate tool for HLA-specific antibody screening in candidate renal transplant recipients able to identify patients with a high risk of poor graft outcome. (literal)
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