Combinatorial peptide ligand libraries for the analysis of low-expression proteins: Validation for normal urine and definition of a first protein MAP. (Articolo in rivista)

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  • Combinatorial peptide ligand libraries for the analysis of low-expression proteins: Validation for normal urine and definition of a first protein MAP. (Articolo in rivista) (literal)
Anno
  • 2012-01-01T00:00:00+01:00 (literal)
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#doi
  • 10.1002/pmic.201100404. (literal)
Alternative label
  • Santucci L, Candiano G, Bruschi M, D'Ambrosio C, Petretto A, Scaloni A, Urbani A, Righetti PG, Ghiggeri GM. (2012)
    Combinatorial peptide ligand libraries for the analysis of low-expression proteins: Validation for normal urine and definition of a first protein MAP.
    in Proteomics (Weinh., Print); Wiley-VCH Verlag Gmbh, Weinheim (Germania)
    (literal)
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#autori
  • Santucci L, Candiano G, Bruschi M, D'Ambrosio C, Petretto A, Scaloni A, Urbani A, Righetti PG, Ghiggeri GM. (literal)
Pagina inizio
  • 509 (literal)
Pagina fine
  • 515 (literal)
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  • 12 (literal)
Rivista
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  • Proteomics. 2012 Jan 13. doi: 10.1002/pmic.201100404. [Epub ahead of print] (literal)
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  • 7 (literal)
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  • 4-5 (literal)
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  • PubMe (literal)
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  • Laboratory of Pathophysiology of Uremia, G. Gaslini Children Hospital, Genoa, Italy National Research Council, ISPAAM, Naples, Italy Laboratory of Experimental and Clinical Immunology - Mass Spectrometry Core Facility, G. Gaslini Children Hospital, Genoa, Italy University of Rome ''Tor Vergata,'' Department of Internal Medicine, Rome, Italy IRCCS-Fondazione Santalucia Centro di Ricerca sul Cervello, Rome, Italy Department of Chemistry, Material and Chemical Engineering ''Giulio Natta,'' Politecnico di Milano, Milan, Italy (literal)
Titolo
  • Combinatorial peptide ligand libraries for the analysis of low-expression proteins: Validation for normal urine and definition of a first protein MAP. (literal)
Abstract
  • In this review, we report the evolution on experimental conditions for the analysis of normal urine based on combinatorial peptide ligand library (CPLL) treatment and successive 2-DE and 2-DE/MS analysis. The main topics are (i) definition of the urine sample requirements, (ii) optimization of the urine/ligand ratio, (iii) essay conditions, (iv) en bloc elution. Overall, normal urine protein composition as studied by 2-DE includes over 2600 spots. Relevant data on inter and intraessay reproducibility obtained by the analysis of different normal urines repeated several times are also here presented. We found a 73% reproducibility upon analysis of the same sample and 68% correspondence of protein composition among different normal urine samples. Based on the above results, we are completing the characterization with LC-MS of 249 spots. The composition of normal urine proteins after CPLLs is finally shown with the indication of those spots which are currently under identification. This map will be completed in a near future; in the meantime this would represent the basic reference sample for newly developed studies on human diseases. (literal)
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