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The 450-band resolution G- and R-banded standard karyotype of the donkey (Equus asinus, 2n = 62). (Articolo in rivista)

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The 450-band resolution G- and R-banded standard karyotype of the donkey (Equus asinus, 2n = 62). (Articolo in rivista) (literal)

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Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#doi

Alternative label

Di Meo GP, Perucatti A, Peretti V, Incarnato D, Ciotola F, Liotta L, Raudsepp T, Di Berardino D, Chowdhary B, Iannuzzi L. (2009)
The 450-band resolution G- and R-banded standard karyotype of the donkey (Equus asinus, 2n = 62).
in Cytogenetic and genome research (Print. ed.); Karger, Basel (Swaziland)
(literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#autori

Di Meo GP, Perucatti A, Peretti V, Incarnato D, Ciotola F, Liotta L, Raudsepp T, Di Berardino D, Chowdhary B, Iannuzzi L. (literal)

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Note

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1. ISPAAM, CNR, Lab Anim Cytogenet & Gene Mapping, IT-80147 Naples, Italy 2. Univ Naples Federico 2, Dept Anim Sci & Food Inspect, Naples, Italy 3. Magna Graecia Univ Catanzaro, Dept Expt & Clin Med, Catanzaro, Italy 4. Univ Messina, Dept MOBIFIPA, Fac Vet Med, Messina, Italy 5. Texas A&M Univ, Coll Vet Med, Dept Vet Integrat Biosci, College Stn, TX 77843 USA 6. Univ Federico II, Dept Soil Plant Environm Sci, Portici, Italy (literal)

Titolo

The 450-band resolution G- and R-banded standard karyotype of the donkey (Equus asinus, 2n = 62). (literal)

Abstract

Donkey chromosomes were earlier characterized separately by C-, G- and R-banding techniques. However, direct comparisons between G- and R-banding patterns have still not been carried out in this species. The present study reports this comparison at the 450-band level by using replication G- and R-banding patterns. Two sets of synchronized lymphocyte cultures were set up to obtain early (GBA+CBA-banding) and late (RBA-banding) BrdU incorporation. Slides were stained with acridine orange and observed under a fluorescence microscope. Reverse GBA+CBA- and RBA-banded karyotypes at the 450-band level were constructed. To verify G- and R-banding patterns in some acrocentric chromosomes, sequential GBA+CBA/Ag-NORs and RBA/Ag-NORs were also performed. The results of CBA-banding patterns obtained in 12 animals from 2 breeds showed a pronounced polymorphism of heterochromatin, especially in EAS1q-prox. Ideogrammatic representations of G- and R-banded karyotypes were constructed using only one common G- and R-banding nomenclature. In the present study both G- and R-banding patterns and relative ideograms are presented as standard karyotype for this species at the 450-band level. (literal)

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