http://www.cnr.it/ontology/cnr/individuo/prodotto/ID51558
X-Y Sperm Aneuploidy in 2 Cattle (Bos taurus) Breeds as Determined by Dual Color Fluorescent in situ Hybridization (FISH) (Articolo in rivista)
- Type
- Label
- X-Y Sperm Aneuploidy in 2 Cattle (Bos taurus) Breeds as Determined by Dual Color Fluorescent in situ Hybridization (FISH) (Articolo in rivista) (literal)
- Anno
- 2009-01-01T00:00:00+01:00 (literal)
- Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#doi
- 10.1159/000245923 (literal)
- Alternative label
D. Nicodemo, A. Pauciullo, A. Castello, E. Roldan, M. Gomendio, G. Cosenza, V. Peretti, A. Perucatti, G.P. Di Meo, L. Ramunno, L. Iannuzzi, J. Rubes, D. Di Berardino (2009)
X-Y Sperm Aneuploidy in 2 Cattle (Bos taurus) Breeds as Determined by Dual Color Fluorescent in situ Hybridization (FISH)
in Cytogenetic and genome research (Print. ed.); Karger, Basel (Swaziland)
(literal)
- Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#autori
- D. Nicodemo, A. Pauciullo, A. Castello, E. Roldan, M. Gomendio, G. Cosenza, V. Peretti, A. Perucatti, G.P. Di Meo, L. Ramunno, L. Iannuzzi, J. Rubes, D. Di Berardino (literal)
- Pagina inizio
- Pagina fine
- Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#url
- http://www.karger.com/Article/FullText/245923 (literal)
- Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#numeroVolume
- Rivista
- Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#pagineTotali
- Note
- ISI Web of Science (WOS) (literal)
- Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#affiliazioni
- Department of Soil, Plant, Environment and Animal Production Sciences, University of Naples 'Federico II', Portici, Italy;
Museo Nacional de Ciencias Naturales, Consejo Superior de Investigaciones Cientificas, Madrid, Spain;
Department of Animal Science and Food Inspection, University of Naples 'Federico II',
Research National Council (CNR), ISPAAM, Laboratory of Animal Cytogenetics and Gene Mapping, Naples, Italy;
Veterinary Research Institute, Brno, Czech Republic (literal)
- Titolo
- X-Y Sperm Aneuploidy in 2 Cattle (Bos taurus) Breeds as Determined by Dual Color Fluorescent in situ Hybridization (FISH) (literal)
- Abstract
- The present study was undertaken to investigate aneuploidy rates in the sperm populations of 2 cattle (Bos taurus) breeds by using dual color fluorescent in situ hybridization (FISH) with Xcen and Y chromosome-specific painting probes, obtained by chromosome microdissection and DOP-PCR. Frozen semen from 10 Italian Friesian and 10 Italian Brown testing bulls was used for the investigation. For each bull, more than 5,000 sperm were analyzed, for a total of 52,586 and 51,342 sperm cells for the 2 breeds, respectively. The present study revealed - in both breeds - a preponderance of the Y-bearing sperm compared to the X-bearing sperm. Within each breed, a statistically significant variation in the various classes of aneuploidy (XX, YY and XY) was found: differences were found in the Friesian breed among the 3 diploidy classes, and in the Brown breed, among the 3 disomy classes (p < 0.05) as well as among the 3 diploidy classes (p < 0.01). However, the 2 breeds did not differ significantly in the overall mean rates of X-Y aneuploidy (disomy + diploidy) which amounts to 0.162% in the Italian Friesian and 0.142% in the Italian Brown. When meiosis I (MI) and II (MII) errors were compared, statistically significant differences (p < 0.01) were found in the disomy classes and in both breeds, whereas the differences between diploidy classes were not significant. Compared to humans, a lower level of aneuploidy has been found in the domestic species analyzed so far. The present study contributes to the establishment of a baseline level of aneuploidy in the sperm populations of 2 cattle breeds which could be used for monitoring future trends of reproductive health, especially in relation to environmental changes and mutagens. (literal)
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- Autore CNR
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