Down-regulation of SM22/transgelin gene expression during H9c2 cells differentiation. (Articolo in rivista)

Type

• Prodotto della ricerca (Classe)
• Articolo in rivista (Classe)

Label

• Down-regulation of SM22/transgelin gene expression during H9c2 cells differentiation. (Articolo in rivista) (literal)

Anno

• 2009-01-01T00:00:00+01:00 (literal)

Alternative label

• Bregant E, Renzone G, Lonigro R, Passon N, Di Loreto C, Pandolfi M, Scaloni A, Tell G, Damante G. (2009)
  Down-regulation of SM22/transgelin gene expression during H9c2 cells differentiation.
  in Molecular and cellular biochemistry
  (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#autori

• Bregant E, Renzone G, Lonigro R, Passon N, Di Loreto C, Pandolfi M, Scaloni A, Tell G, Damante G. (literal)

Pagina inizio

• 145 (literal)

Pagina fine

• 152 (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#numeroVolume

• 327 (literal)

Rivista

• Molecular and cellular biochemistry (Rivista)

Note

• ISI Web of Science (WOS) (literal)

Titolo

• Down-regulation of SM22/transgelin gene expression during H9c2 cells differentiation. (literal)

Abstract

• The embryonic rat ventricle H9c2 cells maintain a proliferative state (P condition) in the presence of 10% FCS. However, by reducing serum concentration and in the presence of retinol acetate, proliferation is stopped, myogenic transdifferentiation is inhibited while cardiac differentiation is preserved (D condition). Two-dimensional gel electrophoresis and mass spectrometry analysis was used to define the modifications of the nuclear proteome occurring during the P-to-D transition. Among the proteins observed as modified, a reduced expression of the SM22/transgelin protein was associated with the D state. Also SM22 mRNA levels were reduced during P-to-D transition. Cell transfection experiments indicated that this decrease was partially due to a reduction of the SM22 promoter activity. GATA-4 had a repressive effect on SM22 promoter activity. Thus, since GATA-4 is known as a target of retinoids and may act as a transcriptional repressor, a mechanism to explain the SM22 reduction during the P-to-D transition is tentatively proposed. Immunohistochemical studies on heart cells confirmed the nuclear localization of SM22. Moreover, a differential expression of this protein in different districts of the human heart embryo was detected. Therefore, these data suggest that SM22 expression is regulated during heart development. (literal)

Prodotto di

• Institute for animal production system in mediterranean environment (ISPAAM) (Istituto)
• Sostenibilità del sistema produzione animale, qualità dei prodotti, tracciabiltà e sicurezza alimentare (AG.P04.013.001) (Modulo)

Autore CNR

• GIOVANNI RENZONE (Unità di personale esterno)
• ANDREA SCALONI (Persona)

Incoming links:

Autore CNR di

• ANDREA SCALONI (Persona)
• GIOVANNI RENZONE (Unità di personale esterno)

Prodotto

• Institute for animal production system in mediterranean environment (ISPAAM) (Istituto)
• Sostenibilità del sistema produzione animale, qualità dei prodotti, tracciabiltà e sicurezza alimentare (AG.P04.013.001) (Modulo)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#rivistaDi

• Molecular and cellular biochemistry (Rivista)