Coexistence of predominantly nonculturable rhizobia with diverse, endophytic bacterial taxa within nodules of wild legumes. (Articolo in rivista)

Type
Label
  • Coexistence of predominantly nonculturable rhizobia with diverse, endophytic bacterial taxa within nodules of wild legumes. (Articolo in rivista) (literal)
Anno
  • 2008-01-01T00:00:00+01:00 (literal)
Alternative label
  • Muresu R, Polone E, Sulas L, Baldan B, Tondello A, Delogu G, Cappuccinelli P, Alberghini S, Benhizia Y, Benhizia H, Benguedouar A, Mori B, Calamassi R, Dazzo FB, Squartini A. (2008)
    Coexistence of predominantly nonculturable rhizobia with diverse, endophytic bacterial taxa within nodules of wild legumes.
    in FEMS microbiology, ecology (print)
    (literal)
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#autori
  • Muresu R, Polone E, Sulas L, Baldan B, Tondello A, Delogu G, Cappuccinelli P, Alberghini S, Benhizia Y, Benhizia H, Benguedouar A, Mori B, Calamassi R, Dazzo FB, Squartini A. (literal)
Pagina inizio
  • 383 (literal)
Pagina fine
  • 400 (literal)
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#numeroVolume
  • 63 (literal)
Rivista
Note
  • ISI Web of Science (WOS) (literal)
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#affiliazioni
  • National Research Council (CNR), ISPAAM, Traversa la Crucca 3, località Baldinca 07100 Li Punti , Sassari, Italy Dipartimento di Biologia, Università di Padova, Via Ugo Bassi 58/b, 35131 Padova, Italy. Dipartimento di Scienze Biomediche, Viale S. Pietro 43 B, 07100 Sassari, Italy. Laboratoire des Biotechnologies, Département des Sciences de la Nature et de la Vie. Université Mentouri, Route de Aïn El Bey, Constantine. Algeria. Laboratoire de Génétique, Biochimie et Biotechnologies Végétales. Département des Sciences de la Nature et de la Vie. Université Mentouri. Route de Aïn El Bey, Constantine. Algeria. Dipartimento di Biologia Vegetale, Università di Firenze. P.le delle Cascine 28, 50144 Firenze, Italy. Department of Microbiology and Molecular Genetics, Michigan State University , East Lansing, MI, 48824 USA. (literal)
Titolo
  • Coexistence of predominantly nonculturable rhizobia with diverse, endophytic bacterial taxa within nodules of wild legumes. (literal)
Abstract
  • A previous analysis showed that Gammaproteobacteria could be the sole recoverable bacteria from surface-sterilized nodules of three wild species of Hedysarum. In this study we extended the analysis to eight Mediterranean native, uninoculated legumes never previously investigated regarding their root-nodule microsymbionts. The structural organization of the nodules was studied by light and electron microscopy, and their bacterial occupants were assessed by combined cultural and molecular approaches. On examination of 100 field-collected nodules, culturable isolates of rhizobia were hardly ever found, whereas over 24 other bacterial taxa were isolated from nodules. None of these nonrhizobial isolates could nodulate the original host when reinoculated in gnotobiotic culture. Despite the inability to culture rhizobial endosymbionts from within the nodules using standard culture media, a direct 16S rRNA gene PCR analysis revealed that most of these nodules contained rhizobia as the predominant population. The presence of nodular endophytes colocalized with rhizobia was verified by immunofluorescence microscopy of nodule sections using an Enterobacter-specific antibody. Hypotheses to explain the nonculturability of rhizobia are presented, and pertinent literature on legume endophytes is discussed. (literal)
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