Discrimination among subterranean clover (Trifolium subterraneum L. complex) genotypes using RAPD markers (Articolo in rivista)

Type
Label
  • Discrimination among subterranean clover (Trifolium subterraneum L. complex) genotypes using RAPD markers (Articolo in rivista) (literal)
Anno
  • 2005-01-01T00:00:00+01:00 (literal)
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#doi
  • 10.1007/s10722-003-4449-6 (literal)
Alternative label
  • Piluzza G.; Pecetti L.; Bullitta S.; Piano E. (2005)
    Discrimination among subterranean clover (Trifolium subterraneum L. complex) genotypes using RAPD markers
    in Genetic resources and crop evolution (Print)
    (literal)
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#autori
  • Piluzza G.; Pecetti L.; Bullitta S.; Piano E. (literal)
Pagina inizio
  • 193 (literal)
Pagina fine
  • 199 (literal)
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#numeroVolume
  • 52 (literal)
Rivista
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#pagineTotali
  • 7 (literal)
Note
  • ISI Web of Science (WOS) (literal)
  • Scopu (literal)
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#affiliazioni
  • ISPAAM-Sezione Pascoli Mediterranei, CNR - Via E. De Nicola, 07100 Sassari, Italy; Istituto Sperimentale per le Colture Foraggere-MiPAAF, Viale Piacenza 29, 26900 Lodi, Italy; (literal)
Titolo
  • Discrimination among subterranean clover (Trifolium subterraneum L. complex) genotypes using RAPD markers (literal)
Abstract
  • RAPD markers were applied to subterranean clover aiming at: (i) assessing the genetic relationships among the subspecies subterraneum L., brachycalycinum Katzn. et Morley, yanninicum Katzn. et Morley, as their taxonomic status is still debated; (ii) verifying the adoption of RAPDs to supplement the common morphological markers used for cultivar identification and protection; (iii) assessing the possible genetic diversity in relation to the geographic origin. Eight primers were selected for genetic analysis of 18 genotypes: 10 subsp. yanninicum (five from Greece and five from Sardinia), six subsp. subterraneum (forming three pairs, each one difficult to distinguish by morphological markers), and two subsp. brachycalycinum. Cluster analysis, performed on the Jaccard's coefficients of association computed across the eight primers, formed three groups of genotypes, corresponding to the three subspecies. The results supported at the DNA level previous inferences, made at cytological, karyological, and isoenzymatic levels, on the ongoing speciation process within the subterranean clover complex, although not warranting yet the full species rank to the three forms. The genotypes of subsp. yanninicum were genetically closer to those of subsp. subterraneum than either group was to the subsp. brachycalycinum genotypes. Within the subsp. yanninicum cluster, the Sardinian genotypes appeared fairly distinct from those from Greece, suggesting a possible, independent evolution going on in different centres of diversity of this subspecies. In two pairs of subsp. subterraneum genotypes, the members could be unequivocally distinguished, thus supporting the role of RAPD fingerprinting in cultivar identification. In the third pair, the two genotypes appeared to be the same, inadvertently duplicated within the germplasm collection. (literal)
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