Selective ion tracing and msn analysis of peptide digests from fsba-treated kinases for the analysis of protein atp-binding sites (Articolo in rivista)

Type
Label
  • Selective ion tracing and msn analysis of peptide digests from fsba-treated kinases for the analysis of protein atp-binding sites (Articolo in rivista) (literal)
Anno
  • 2006-01-01T00:00:00+01:00 (literal)
Alternative label
  • Renzone G, Salzano AM, Arena S, D'Ambrosio C, Scaloni A (2006)
    Selective ion tracing and msn analysis of peptide digests from fsba-treated kinases for the analysis of protein atp-binding sites
    in Journal of proteome research (Print)
    (literal)
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  • Renzone G, Salzano AM, Arena S, D'Ambrosio C, Scaloni A (literal)
Pagina inizio
  • 2019 (literal)
Pagina fine
  • 2024 (literal)
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  • 5 (literal)
Rivista
Note
  • ISI Web of Science (WOS) (literal)
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#affiliazioni
  • Proteomics & Mass Spectrometry Laboratory, ISPAAM, National Research Council, 80147 Naples, Italy. (literal)
Titolo
  • Selective ion tracing and msn analysis of peptide digests from fsba-treated kinases for the analysis of protein atp-binding sites (literal)
Abstract
  • Kinases play a key role in many cellular processes by catalyzing the transfer of phosphoryl groups from ATP to a broad number of substrates, including amino acids on target proteins. The reagent 5'-fluorosulfonylbenzoyl-5'-adenosine (FSBA) has been widely used to identify ATP-binding sites in kinases since it reacts with nucleophilic amino acids occurring within these motifs, determining a mass increase of 433 Da. In this study, we present a versatile MS approach that has been developed to recognize labeled peptides generated after enzymatic digestion of FSBA-treated kinases. Using selective ion tracing and MS(2)/MS(3) experiments, we were able to easily identify peptides occurring at protein ATP-binding sites, also affording a complete characterization of the modified amino acids. This methodology may be used in the development of future parent ion scanning-based applications directed to large scale analysis of kinases within complex protein mixtures. (literal)
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