DNA prime and protein boost immunization with innovative polymeric cationic core-shell nanoparticles elicits broad immune responses and strongly enhance cellular responses of HIV-1 tat DNA vaccination (Articolo in rivista)

Type

• Prodotto della ricerca (Classe)
• Articolo in rivista (Classe)

Label

• DNA prime and protein boost immunization with innovative polymeric cationic core-shell nanoparticles elicits broad immune responses and strongly enhance cellular responses of HIV-1 tat DNA vaccination (Articolo in rivista) (literal)

Anno

• 2006-01-01T00:00:00+01:00 (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#doi

• 10.1016/j.vaccine.2006.05.058 (literal)

Alternative label

• A. Castaldello, E. Brocca Cofano, R. Voltan, C. Triulzi, G. Altavilla, M. Laus, K. Sparnacci, M. Ballestri, L. Tondelli, C. Fortini, R. Gavioli, B. Ensoli (2006)
  DNA prime and protein boost immunization with innovative polymeric cationic core-shell nanoparticles elicits broad immune responses and strongly enhance cellular responses of HIV-1 tat DNA vaccination
  in Vaccine
  (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#autori

• A. Castaldello, E. Brocca Cofano, R. Voltan, C. Triulzi, G. Altavilla, M. Laus, K. Sparnacci, M. Ballestri, L. Tondelli, C. Fortini, R. Gavioli, B. Ensoli (literal)

Pagina inizio

• 5655 (literal)

Pagina fine

• 5669 (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#numeroVolume

• 24 (literal)

Rivista

• Vaccine (Rivista)

Note

• ISI Web of Science (WOS) (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#affiliazioni

• 1. Univ Padua, Microbiol Sect, Dept Histol Microbiol & Med Biotechnol, I-35122 Padua, Italy 2. Univ Ferrara, Dept Biochem & Mol Biol, I-44100 Ferrara, Italy 3. Univ Padua, Dept Pathol Anat & Histol, I-35122 Padua, Italy 4. Univ Piemonte Orientale, Dept Life & Ambient Sci, Alessandria, Italy 5. INSTM, UdR, Alessandria, Italy 6. CNR, ISOF, I-40126 Bologna, Italy 7. Ist Super Sanita, Natl AIDS Ctr, I-00161 Rome, Italy (literal)

Titolo

• DNA prime and protein boost immunization with innovative polymeric cationic core-shell nanoparticles elicits broad immune responses and strongly enhance cellular responses of HIV-1 tat DNA vaccination (literal)

Abstract

• Novel biocompatible core-shell cationic nanoparticles, composed of an inner hard core of poly(methylmethacrylate) (PMMA) and a hydrophilic tentacular shell bearing positively charged groups and poly(ethyleneglycol) chains covalently bound to the core, were prepared by emulsion polymerization and characterized in vitro and in vivo for DNA vaccine applications. The nanoparticles reversibly adsorbed large amounts of DNA, mainly through electrostatic interactions, preserved its functional structure, efficiently delivered it intracellularly, and were not toxic in vitro or in mice. Furthermore, two intramuscular (i.m.) immunizations (4 weeks apart) with a very low dose (1 mu g) of the plasmid pCV-tat delivered by these nanoparticles followed by one or two protein boosts induced significant antigen-specific humoral and cellular responses and greatly increased Th1-type T cell responses and CTLs against HIV-1 Tat. (c) 2006 Elsevier Ltd. All rights reserved. (literal)

Prodotto di

• Institute for organic syntheses and photoreactivity (ISOF) (Istituto)
• Nuovi metodi per lo sviluppo e la caratterizzazione di materiali polimerici ottenuti con sintesi non convenzionali (PM.P02.006.001) (Modulo)

Autore CNR

• LUISA TONDELLI (Persona)
• MARCO BALLESTRI (Persona)

Incoming links:

Prodotto

• Institute for organic syntheses and photoreactivity (ISOF) (Istituto)
• Nuovi metodi per lo sviluppo e la caratterizzazione di materiali polimerici ottenuti con sintesi non convenzionali (PM.P02.006.001) (Modulo)

Autore CNR di

• LUISA TONDELLI (Persona)
• MARCO BALLESTRI (Persona)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#rivistaDi

• Vaccine (Rivista)