http://www.cnr.it/ontology/cnr/individuo/prodotto/ID50793
Investigation of Radical-Based Damage of RNase A in Aqueous Solutions and Lipid Vesicles (Articolo in rivista)
- Type
- Label
- Investigation of Radical-Based Damage of RNase A in Aqueous Solutions and Lipid Vesicles (Articolo in rivista) (literal)
- Anno
- 2006-01-01T00:00:00+01:00 (literal)
- Alternative label
Torreggiani, A.(1); Tamba, M.(1); Manco, I.(1); Faraone-Mennella(2), M. R.; Ferreri, C.(1); Chatgilialoglu, C(1). (2006)
Investigation of Radical-Based Damage of RNase A in Aqueous Solutions and Lipid Vesicles
in Biopolymers (Print)
(literal)
- Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#autori
- Torreggiani, A.(1); Tamba, M.(1); Manco, I.(1); Faraone-Mennella(2), M. R.; Ferreri, C.(1); Chatgilialoglu, C(1). (literal)
- Pagina inizio
- Pagina fine
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- Rivista
- Note
- ISI Web of Science (WOS) (literal)
- Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#affiliazioni
- 1. Istituto I.S.O.F., Consiglio
Nazionale delle Ricerche,
Via P. Gobetti 101,
40129 Bologna, Italy
2. Dipartimento di Chimica
Biologica, Universita` di Napoli,
via Mezzocannone 16,
80134 Napoli, Italy (literal)
- Titolo
- Investigation of Radical-Based Damage of RNase A in Aqueous Solutions and Lipid Vesicles (literal)
- Abstract
- The gamma-irradiation of bovine pancreatic ribonuclease A (RNase A) in aqueous solution
were investigated at different doses by vibrational spectroscopy as well as enzymatic assay, electrophoresis,
and HPLC analysis. Both functional and structural changes of the protein were caused by
attack of H? atoms and ?OH radicals. In particular, Raman spectroscopy was shown to be a useful
tool in identifying conformational changes of the protein structure and amino acidic residues that
are preferential sites of the radical attack (i.e., tyrosine and methionine). After partial structural
changes by the initial radical attack, the internal sulfur-containing amino acid residues were rendered
susceptible to transformation. By using the biomimetic model of dioleoyl phosphatidyl choline
vesicle suspensions containing RNase A, the damage to methione residues could be connected to a
parallel alteration of membrane unsaturated lipids. In fact, thiyl radical species formed from protein
degradation can diffuse into the lipid bilayer and cause isomerization of the naturally occurring
cis double bonds. As a consequence, trans unsaturated fatty acids are formed in vesicles and
can be considered to be markers of this protein damage. (literal)
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