Radiation Damage of Lysozyme in a Biomimetic Model: Some Insights by Raman Spectroscopy (Articolo in rivista)

Type

• Articolo in rivista (Classe)
• Prodotto della ricerca (Classe)

Label

• Radiation Damage of Lysozyme in a Biomimetic Model: Some Insights by Raman Spectroscopy (Articolo in rivista) (literal)

Anno

• 2005-01-01T00:00:00+01:00 (literal)

Alternative label

• Ferreri, Carla (1); Torreggiani, Armida (1); CHATGILIALOGLU, CHRYSSOSTOMOS (1); TAMBA, MAURIZIO (1); Faraone-Mennella, Maria Rosaria (2); Manco, Immacolata (2) (2005)
  Radiation Damage of Lysozyme in a Biomimetic Model: Some Insights by Raman Spectroscopy
  in Journal of molecular structure (Print)
  (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#autori

• Ferreri, Carla (1); Torreggiani, Armida (1); CHATGILIALOGLU, CHRYSSOSTOMOS (1); TAMBA, MAURIZIO (1); Faraone-Mennella, Maria Rosaria (2); Manco, Immacolata (2) (literal)

Pagina inizio

• 767 (literal)

Pagina fine

• 773 (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#numeroVolume

• - (literal)

Rivista

• Journal of molecular structure (Rivista)

Note

• ISI Web of Science (WOS) (literal)
• Scopu (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#affiliazioni

• (1) Istituto ISOF - Consiglio Nazionale delle Ricerche; (2) Dip. Chimica Biologica - Università di Napoli (literal)

Titolo

• Radiation Damage of Lysozyme in a Biomimetic Model: Some Insights by Raman Spectroscopy (literal)

Abstract

• A biomimetic model for protein/lipid radical damage is described. The model shows that the protein degradation is accompanied by trans lipid isomerization due to thiyl radicals, derived from sulfur-containing residues and diffused through the lipid bilayer. ?-Irradiation was used to obtain free radical generation and lysozyme was chosen as enzyme example. A detailed study of the Raman spectra of lysozyme irradiated at different doses coupled to vesicle experiments, as well as enzymatic assays, is reported to gather a comprehensive view of the irradiation effect. The lysozyme resistance to degradation by ?-irradiation is remarkable and the protein structure play a significant role in blocking the ready access of free radicals both to the sulfur-containing residues and the active site. The degradation of sulfur moieties does not occur immediately, but as irradiation progressed, the involvement of these residues takes place. In fact, structural changes induced on the protein render these residues more exposed and susceptible of radical attack. Tyr are confirmed to be among the most sensitive residues towards oxidation. These results can be relevant for a better understanding of mechanisms of tandem radical damages occurring in a biological environment. (literal)

Prodotto di

• Indagine genomica correlata al danneggiamento del DNA provocato da attacchi radicalici (PM.P06.006.002) (Modulo)
• Institute for organic syntheses and photoreactivity (ISOF) (Istituto)
• Modelli biomimetici di stress radicalico e biomarcatori correlati (PM.P06.006.001) (Modulo)

Autore CNR

• MAURIZIO TAMBA (Unità di personale interno)
• ARMIDA TORREGGIANI (Unità di personale interno)
• CARLA FERRERI (Unità di personale interno)
• CHRYSSOSTOMOS CHATGILIALOGLU (Unità di personale interno)

Insieme di parole chiave

• Keywords of "Radiation Damage of Lysozyme in a Biomimetic Model: Some Insights by Raman Spectroscopy" (Insieme di parole chiave)

Incoming links:

Prodotto

• Institute for organic syntheses and photoreactivity (ISOF) (Istituto)
• Modelli biomimetici di stress radicalico e biomarcatori correlati (PM.P06.006.001) (Modulo)
• Indagine genomica correlata al danneggiamento del DNA provocato da attacchi radicalici (PM.P06.006.002) (Modulo)

Autore CNR di

• CARLA FERRERI (Unità di personale interno)
• CHRYSSOSTOMOS CHATGILIALOGLU (Unità di personale interno)
• MAURIZIO TAMBA (Unità di personale interno)
• ARMIDA TORREGGIANI (Unità di personale interno)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#rivistaDi

• Journal of molecular structure (Rivista)

Insieme di parole chiave di

• Keywords of "Radiation Damage of Lysozyme in a Biomimetic Model: Some Insights by Raman Spectroscopy" (Insieme di parole chiave)