Selective expression of TLQP-21 and other VGF peptides in gastric neuroendocrine cells and modulation by feeding (Articolo in rivista)

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Label
  • Selective expression of TLQP-21 and other VGF peptides in gastric neuroendocrine cells and modulation by feeding (Articolo in rivista) (literal)
Anno
  • 2010-01-01T00:00:00+01:00 (literal)
Alternative label
  • Brancia C.1, Cocco C.1, D'Amato F.1, Noli B.1, Sanna F.2, Possenti R.3, Argiolas A.2, Ferri G.L.1 (2010)
    Selective expression of TLQP-21 and other VGF peptides in gastric neuroendocrine cells and modulation by feeding
    in Journal of Endocrinology
    (literal)
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#autori
  • Brancia C.1, Cocco C.1, D'Amato F.1, Noli B.1, Sanna F.2, Possenti R.3, Argiolas A.2, Ferri G.L.1 (literal)
Pagina inizio
  • 329 (literal)
Pagina fine
  • 341 (literal)
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  • Epub 2010 Sep 27. (literal)
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  • 207 (literal)
Rivista
Note
  • ISI Web of Science (WOS) (literal)
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  • 1 - NEF-Laboratory, Department of Cytomorphology, e 2 - Department of Neuroscience, University of Cagliari, 09042 Monserrato (Cagliari), Italy. 3 - Institute of Neurobiology and Molecular Medicine, CNR, e Department of Neuroscience, Tor Vergata University, 00133 Rome, Italy. (literal)
Titolo
  • Selective expression of TLQP-21 and other VGF peptides in gastric neuroendocrine cells and modulation by feeding (literal)
Abstract
  • Although vgf gene knockout mice are hypermetabolic, administration of the VGF peptide TLQP-21 itself increased energy consumption. Agonist-antagonist roles are thus suggested for different VGF peptides, and the definition of their tissue heterogeneity is mandatory. We studied the rat stomach using antisera to C- or N-terminal sequences of known or predicted VGF peptides in immunohistochemistry and ELISA. TLQP (rat VGF(556-565)) peptide/s were most abundant (162±11 pmol/g, mean±s.e.m.) and were brightly immunostained in enterochromaffin-like (ECL) cells and somatostatin cells. A peptide co-eluting with TLQP-21 was revealed in HPLC of gastric and hypothalamic extracts, while the extended TLQP-62 form was restricted to the hypothalamus. Novel PGH (rat VGF(422-430)) peptide/s were revealed in ghrelin cells, mostly corresponding to low MW forms (0.8-1.5 kDa), while VGF C-terminus peptides were confined to neurons. VGF mRNA was present in the above gastric endocrine cell types, and was prominent in chief cells, in parallel with low-intensity staining for further cleaved products from the C-terminal region of VGF (HVLL peptides: VGF(605-614)). In swine stomach, a comparable profile of VGF peptides was revealed by immunohistochemistry. When fed and fasted rats were studied, a clear-cut, selective decrease on fasting was observed for TLQP peptides only (162±11 vs 74±5.3 pmol/g, fed versus fasted rats, mean±s.e.m., P<0.00001). In conclusion, specific VGF peptides appear to be widely represented in different gastric endocrine and other mucosal cell populations. The selective modulation of TLQP peptides suggests their involvement in peripheral neuro-endocrine mechanisms related to feeding responses and/or ECL cell regulation. (literal)
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