Novel biocompatible anionic polymeric microspheres for the delivery of the HIV-tat protein for vaccine application (Articolo in rivista)

Type

• Articolo in rivista (Classe)
• Prodotto della ricerca (Classe)

Label

• Novel biocompatible anionic polymeric microspheres for the delivery of the HIV-tat protein for vaccine application (Articolo in rivista) (literal)

Anno

• 2004-01-01T00:00:00+01:00 (literal)

Alternative label

• A.Caputo, E. Brocca-Cofano, A. Castaldello, R. De Michele, G. Altavilla, M. Marchisio, R. Gavioli, U. Rolen, L. Chiarantini, A. Cerasi, S. Dominici, M. Magnani, A. Cafaro, K. Sparnacci, M. Laus, L. Tondelli, B. Ensoli (2004)
  Novel biocompatible anionic polymeric microspheres for the delivery of the HIV-tat protein for vaccine application
  in Vaccine
  (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#autori

• A.Caputo, E. Brocca-Cofano, A. Castaldello, R. De Michele, G. Altavilla, M. Marchisio, R. Gavioli, U. Rolen, L. Chiarantini, A. Cerasi, S. Dominici, M. Magnani, A. Cafaro, K. Sparnacci, M. Laus, L. Tondelli, B. Ensoli (literal)

Pagina inizio

• 2910 (literal)

Pagina fine

• 2924 (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#numeroVolume

• 21-22 (literal)

Rivista

• Vaccine (Rivista)

Note

• ISI Web of Science (WOS) (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#affiliazioni

• 1. Univ Ferrara, Dept Expt & Diagnost Med, Microbiol Sect, I-44100 Ferrara, Italy 2. Univ Padua, Inst Pathol Anat & Histol, I-35100 Padua, Italy 3. Univ Ferrara, Dept Morphol & Embryol, I-44100 Ferrara, Italy 4. Univ Ferrara, Dept Biochem & Mol Biol, I-44100 Ferrara, Italy 5. Univ Urbino, Dept Biochem, I-61029 Urbino, Italy 6. Ist Super Sanita, Virol Lab, I-00161 Rome, Italy 7. Univ Piemonte Orientale, Dept Adv Sci & Technol, I-15100 Alessandria, Italy 8. UdR, INSTM, I-15100 Alessandria, Italy 9. CNR, ISOF, I-40129 Bologna, Italy (literal)

Titolo

• Novel biocompatible anionic polymeric microspheres for the delivery of the HIV-tat protein for vaccine application (literal)

Abstract

• Two novel classes of biocompatible core-shell anionic microspheres, composed of an inner hard insoluble core, either made of poly(styrene) (PS) or poly(methyl methacrylate) (PMMA), and a soft outer tentacular shell made of long soluble negatively charged arms derived from the steric stabilizer, hemisuccinated poly(vinyl alcohol) or Eudragit L100/55, respectively, were prepared by dispersion polymerization and characterized. Five types of these novel microspheres, two made of poly(styrene) and hemisuccinated poly(vinyl alcohol) (A4 and A7), and three made of poly(methyl methacrylate) and Eudragit L100/55 (1D, 1E, H1D), differing for chemical composition, size, and surface charge density were analyzed for the delivery of the HIV-1 Tat protein for vaccine applications. All microspheres reversibly adsorbed the native biologically active HIV-1 Tat protein preventing Tat from oxidation and maintaining its biological activity, therefore increasing the shelf-life of the Tat protein vaccine. The microspheres efficiently delivered Tat intracellularly, and were not toxic in vitro nor in mice, even after multiple administrations. These results indicate that these novel microparticles are safe and represent a promising delivery system for vaccination with Tat, as well as for other subunit vaccines, particularly when a native protein conformation is required. (literal)

Prodotto di

• Institute for organic syntheses and photoreactivity (ISOF) (Istituto)
• Nuovi metodi per lo sviluppo e la caratterizzazione di materiali polimerici ottenuti con sintesi non convenzionali (PM.P02.006.001) (Modulo)

Autore CNR

• LUISA TONDELLI (Unità di personale interno)

Incoming links:

Prodotto

• Institute for organic syntheses and photoreactivity (ISOF) (Istituto)
• Nuovi metodi per lo sviluppo e la caratterizzazione di materiali polimerici ottenuti con sintesi non convenzionali (PM.P02.006.001) (Modulo)

Autore CNR di

• LUISA TONDELLI (Unità di personale interno)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#rivistaDi

• Vaccine (Rivista)