Olfactory ensheathing cells exert a trophic effect on the hypothalamic neurons “in vitro”. (Articolo in rivista)

Type

• Prodotto della ricerca (Classe)
• Articolo in rivista (Classe)

Label

• Olfactory ensheathing cells exert a trophic effect on the hypothalamic neurons “in vitro”. (Articolo in rivista) (literal)

Anno

• 2007-01-01T00:00:00+01:00 (literal)

Alternative label

• R.Pellitteri, M. Spatuzza, A. Russo, S. Stanzani (2007)
  Olfactory ensheathing cells exert a trophic effect on the hypothalamic neurons “in vitro”.
  in Neuroscience letters (Print)
  (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#autori

• R.Pellitteri, M. Spatuzza, A. Russo, S. Stanzani (literal)

Pagina inizio

• 24 (literal)

Pagina fine

• 29 (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#numeroVolume

• 417 (literal)

Rivista

• Neuroscience letters (Rivista)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#descrizioneSinteticaDelProdotto

• In this study, we developed an in vitro model to evaluate the neurotrophic effect of OECs on the survival and axonal outgrowth of hypothalamic neurons. Co-cultures of OECs and hypothalamus neuronal cells of postnatal rats were successfully established and cells were immunocytochemically characterized. Furthermore, some neuronal cultures were added with NGF, bFGF and GDNF to compare with the co-cultures. Our results indicate that in co-cultures of hypothalamic neurons and OECs, the number of neurons was significantly increased compared to control cultures exhibiting a dense axonal outgrowth. Moreover, we show that NGF promoted a major neuronal survival than bFGF and GDNF, while bFGF and GDNF exerted an evidence axonal and dendritic outgrowth compared to NGF. In conclusion, these data suggest that OECs have the capacity to promote the survival and axonal outgrowth of hypothalamic neurons in vitro and that bFGF, NGF and GDNF differentially support hypothalamic neurons promoting and enhancing the neuronal survival and outgrowth. (literal)

Note

• ISI Web of Science (WOS) (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#affiliazioni

• 1Institute of Neurological Sciences, National Research Council, Section of Catania, viale R. Margherita 6, 95123 Catania, Italy, 2Department of Physiological Science, University of Catania, viale A. Doria 6, 95125 Catania, Italy (literal)

Titolo

• Olfactory ensheathing cells exert a trophic effect on the hypothalamic neurons “in vitro”. (literal)

Abstract

• Olfactory ensheathing cells (OECs) constitute an usual population of glial cells sharing properties with both Schwann cells of peripheral nervous system (PNS) and astrocytes of the central nervous system (CNS). They express a high level of growth factors which play a very important role as neuronal support. Recent evidence in literature suggests that OECs may facilitate axonal regeneration in the injured nervous system. In this study, we developed an in vitro model to evaluate the neurotrophic effect of OECs on the survival and axonal outgrowth of hypothalamic neurons. Co-cultures of OECs and hypothalamus neuronal cells of postnatal rats were successfully established and cells were immunocytochemically characterized. Furthermore, some neuronal cultures were added with NGF, bFGF and GDNF to compare with the co-cultures. Our results indicate that in co-cultures of hypothalamic neurons and OECs, the number of neurons was significantly increased compared to control cultures exhibiting a dense axonal outgrowth. Moreover, we show that NGF promoted a major neuronal survival than bFGF and GDNF, while bFGF and GDNF exerted an evidence axonal and dendritic outgrowth compared to NGF. In conclusion, these data suggest that OECs have the capacity to promote the survival and axonal outgrowth of hypothalamic neurons in vitro and that bFGF, NGF and GDNF differentially support hypothalamic neurons promoting and enhancing the neuronal survival and outgrowth. Therefore, the OECs are a source of growth factors and might be considered a better approach for functional recovery and growth factors might exert a neuroprotective effect in neurodegenerative disorders. (literal)

Prodotto di

• Institute of neurological sciences (ISN) (Istituto)
• Markers molecolari nelle malattie ereditarie e tumori del Sistema Nervoso (ME.P02.011.001) (Modulo)

Autore CNR

• MICHELA SPATUZZA (Unità di personale esterno)
• ROSALIA MARIA CRISTINA PELLITTERI (Unità di personale interno)

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• Keywords of "Olfactory ensheathing cells exert a trophic effect on the hypothalamic neurons “in vitro”." (Insieme di parole chiave)

Incoming links:

Autore CNR di

• ROSALIA MARIA CRISTINA PELLITTERI (Unità di personale interno)
• MICHELA SPATUZZA (Unità di personale esterno)

Prodotto

• Institute of neurological sciences (ISN) (Istituto)
• Markers molecolari nelle malattie ereditarie e tumori del Sistema Nervoso (ME.P02.011.001) (Modulo)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#rivistaDi

• Neuroscience letters (Rivista)

Insieme di parole chiave di

• Keywords of "Olfactory ensheathing cells exert a trophic effect on the hypothalamic neurons “in vitro”." (Insieme di parole chiave)