http://www.cnr.it/ontology/cnr/individuo/prodotto/ID4983

In vitro and in vivo pharmacological role of TLQP-21, a VGF-derived peptide, in the regulation of rat gastric motor functions (Articolo in rivista)

Type

Prodotto della ricerca (Classe)
Articolo in rivista (Classe)

Label

In vitro and in vivo pharmacological role of TLQP-21, a VGF-derived peptide, in the regulation of rat gastric motor functions (Articolo in rivista) (literal)

Anno

2009-01-01T00:00:00+01:00 (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#doi

10.1111/j.1476-5381.2009.00192.x (literal)

Alternative label

Severini C. 1 ; La Corte G. 1 ; Improta G. 2 ; Broccardo M. 2 ; Agostini S. 2 ; Petrella C. 2 ; Sibilia V. 3 ; Pagani F. 3; Guidobono F. 3 ; Bulgarelli I. 4 ; Ferri G.L. 5 ; Brancia C. 5 ; Rinaldi A.M. 6 ; Levi A. 1 ; Possenti R. 1,6 (2009)
In vitro and in vivo pharmacological role of TLQP-21, a VGF-derived peptide, in the regulation of rat gastric motor functions
in British journal of pharmacology
(literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#autori

Severini C. 1 ; La Corte G. 1 ; Improta G. 2 ; Broccardo M. 2 ; Agostini S. 2 ; Petrella C. 2 ; Sibilia V. 3 ; Pagani F. 3; Guidobono F. 3 ; Bulgarelli I. 4 ; Ferri G.L. 5 ; Brancia C. 5 ; Rinaldi A.M. 6 ; Levi A. 1 ; Possenti R. 1,6 (literal)

Pagina inizio

984 (literal)

Pagina fine

993 (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#altreInformazioni

Epub 2009 May 19. IF (ISI WOS 2009)= 5.204 SRJ SCImago (2009)= 1,692 (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#numeroVolume

157 (literal)

Rivista

British journal of pharmacology (Rivista)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#numeroFascicolo

6 (literal)

Note

Scopu (literal)
ISI Web of Science (WOS) (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#affiliazioni

1 - Institute of Neurobiology and Molecular Medicine, CNR, Via del Fosso di Fiorano 64/65, Rome, Italy; 2 - Department of Human Physiology and Pharmacology, 'V.Erspamer', University 'La Sapienza', Rome, Italy; 3 - Department of Pharmacology, Chemotherapy and Medical Toxicology, University of Milan, Milan, Italy; 4 - Department of Experimental and Environmental Medicine and Biotechnology, University of 'Milano-Bicocca', Milan, Italy; 5 -NEF-Laboratory, Department of Cytomorphology, University of Cagliari, Cagliari, Italy, 6 - Department of Neuroscience, University 'Tor Vergata' and Fondazione Santa Lucia, Rome, Italy (literal)

Titolo

In vitro and in vivo pharmacological role of TLQP-21, a VGF-derived peptide, in the regulation of rat gastric motor functions (literal)

Abstract

BACKGROUND AND PURPOSE: Vgf gene expression has been detected in various endocrine and neuronal cells in the gastrointestinal tract. In this study we investigated the pharmacological activity of different VGF-derived peptides. Among these, TLQP-21, corresponding to the 556-576 fragment of the protein was the unique active peptide, and its pharmacological profile was further studied. EXPERIMENTAL APPROACH: The effects of TLQP-21 were examined in vitro by smooth muscle contraction in isolated preparations from the rat gastrointestinal tract and, in vivo, by assessing gastric emptying in rats. Rat stomach tissues were also processed for immunohistochemical and biochemical characterization. KEY RESULTS: In rat longitudinal forestomach strips, TLQP-21 (100 nmol x L(-1)-10 micromol x L(-1)) concentration-dependently induced muscle contraction (in female rats, EC(50) = 0.47 micromol.L(-1), E(max): 85.7 +/- 7.9 and in male rats, 0.87 micromol x L(-1), E(max): 33.4 +/- 5.3; n = 8), by release of prostaglandin (PG)E(2) and PGF(2a) from the mucosal layer. This effect was significantly antagonized by indomethacin and selective inhibitors of either cyclooxygenase-1 (S560) or cyclooxygenase-2 (NS398). Immunostaining and biochemical studies confirmed the presence of VGF in the gastric neuronal cells. TLQP-21, injected i.c.v. (2-32 nmol per rat), significantly decreased gastric emptying by about 40%. This effect was significantly (P < 0.05) blocked by i.c.v. injection of indomethacin, suggesting that, also in vivo, this peptide acts in the brain stimulating PG release. CONCLUSIONS AND IMPLICATIONS: The present results demonstrate that this VGF-derived peptide plays a central and local role in the regulation of rat gastric motor functions. (literal)

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