http://www.cnr.it/ontology/cnr/individuo/prodotto/ID4979

MicroRNA-92 modulates K(+) Cl(-) co-transporter KCC2 expression in cerebellar granule neurons (Articolo in rivista)

Type

Label

MicroRNA-92 modulates K(+) Cl(-) co-transporter KCC2 expression in cerebellar granule neurons (Articolo in rivista) (literal)

Anno

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#doi

Alternative label

Barbato C. 1, 2, Ruberti F. 1, Pieri M. 3,4, Vilardo E. 1, Costanzo M. 1, Ciotti M.T. 1, Zona C. 3,4, Cogoni C. 1,5 (2010)
MicroRNA-92 modulates K(+) Cl(-) co-transporter KCC2 expression in cerebellar granule neurons
in Journal of neurochemistry; John Wiley & Sons Inc., Hoboken (Stati Uniti d'America)
(literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#autori

Barbato C. 1, 2, Ruberti F. 1, Pieri M. 3,4, Vilardo E. 1, Costanzo M. 1, Ciotti M.T. 1, Zona C. 3,4, Cogoni C. 1,5 (literal)

Pagina inizio

Pagina fine

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#altreInformazioni

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#url

http://onlinelibrary.wiley.com/doi/10.1111/j.1471-4159.2009.06560.x/abstract (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#numeroVolume

Rivista

Note

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#affiliazioni

1 - EBRI European Brain Research Institute Fondazione EBRI Rita Levi-Montalcini, Rome, Italy; 2 - INMM Istituto di Neurobiologia e Medicina Molecolare, CNR, Rome, Italy; 3 - Department of Neuroscience, University of Rome 'Tor Vergata', Rome, Italy; 4 - Fondazione S. Lucia, I.R.C.C.S., Rome, Italy; 5 - Dipartimento di Biotecnologie Cellulari ed Ematologia, Università di Roma 'La Sapienza', Rome, Italy Address correspondence and reprint requests to Francesca Ruberti, INMM Istituto di Neurobiologia e (literal)

Titolo

MicroRNA-92 modulates K(+) Cl(-) co-transporter KCC2 expression in cerebellar granule neurons (literal)

Abstract

Abstract MicroRNAs have been associated to fine-tuning spatial and temporal control of gene expression during neuronal development. The neuronal Cl(-) extruding, K(+)Cl(-) co-transporter 2 (KCC2) is known to play an important role in neuronal Cl(-) homeostasis and in determining the physiological response to activation of anion selective GABA receptors. Here we show that microRNA-92 is developmentally down-regulated during maturation of rat cerebellar granule neurons (CGNs) in vitro. Computational predictions suggest several high-ranking targets for microRNA-92 including the KCC2 gene. Consistently, the KCC2 protein levels were up-regulated in mature CGN in vitro and a functional association between microRNA-92 and KCC2 3' untranslated region was established using luciferase assays. The generation of an inward directed Cl(-) electrochemical gradient, necessary for the hyperpolarizing effect of GABA, requires robust KCC2 expression in several neuronal types. Here we show that lentiviral-mediated microRNA-92 over-expression reduced KCC2 protein levels and positively shifted reversal potential of GABA induced Cl(-) currents in CGNs. In addition KCC2 re-expression reversed microRNA-92 electrophysiological phenotype. Consistently microRNA-92 inhibition induced both an increase of the level of KCC2 and a negative shift in GABA reversal potential. These findings introduce a new player in the developmental change of GABA from depolarization to hyperpolarization. (literal)

Editore