Detection of microalgal resting cysts in European coastal sediments using a PCR-based assay (Articolo in rivista)

Type
Label
  • Detection of microalgal resting cysts in European coastal sediments using a PCR-based assay (Articolo in rivista) (literal)
Anno
  • 2010-01-01T00:00:00+01:00 (literal)
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#doi
  • 10.1016/j.dsr2.2009.09.010 (literal)
Alternative label
  • Penna A; Battocchi C; Garces E; Angles S; Cucchiari E; Totti C; Kremp A; Satta C; Giacobbe MG; Bravo I; Bastianini M (2010)
    Detection of microalgal resting cysts in European coastal sediments using a PCR-based assay
    in Deep-sea research. Part 2. Topical studies in oceanography
    (literal)
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#autori
  • Penna A; Battocchi C; Garces E; Angles S; Cucchiari E; Totti C; Kremp A; Satta C; Giacobbe MG; Bravo I; Bastianini M (literal)
Pagina inizio
  • 288 (literal)
Pagina fine
  • 300 (literal)
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#numeroVolume
  • 57 (literal)
Rivista
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#numeroFascicolo
  • 3-4 (literal)
Note
  • ISI Web of Science (WOS) (literal)
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#affiliazioni
  • Penna, Antonella Dipartimento di Scienze Biomolecolari, Università di Urbino, Viale Trieste 296, 61100 Pesaro, Italy Battocchi, Cecilia Dipartimento di Scienze Biomolecolari, Università di Urbino, Viale Trieste 296, 61100 Pesaro, Italy Garces, Esther CSIC, ICM, Inst Marine Sci, Barcelona 08002, Spain Angles, Silvia CSIC, ICM, Inst Marine Sci, Barcelona 08002, Spain Cucchiari, Emelina Univ Politecn Marche, Dept Marine Sci, Ancona, Italy Totti, Cecilia Univ Politecn Marche, Dept Marine Sci, Ancona, Italy Kremp, Anke Tvarminne Zoological Station, University of Helsinki, 10900 Hanko, Finland Satta, Cecilia Dip. Botanica ed Ecologia Vegetale, University of Sassari, 07100 Sassari, Italy Giacobbe, Maria Grazia CNR-IAMC, Messina Bravo, Isabel Instituto Espan~ol de Oceanograf?´a, Apdo. 1552, 36200 Vigo, Spain Bastianini, Mauro CNR-ISMAR, Venezia (literal)
Titolo
  • Detection of microalgal resting cysts in European coastal sediments using a PCR-based assay (literal)
Abstract
  • A PCR-based assay was developed and applied to sediment and sediment trap samples for the detection of different cysts belonging to dinoflagellates and raphidophytes in European coastal areas. Oligonucleotide primers were designed based on the ITS-5.8S and LSU ribosomal gene sequences. The specificity and sensitivity of the PCR assay were assessed using genomic DNA from clonal cultures, plasmid copy number of cloned target sequences, as well as from sediment samples. Qualitative PCR determinations of different cysts in sediment and sediment trap samples were compared to taxonomic examinations by light microscopy. This molecular methodology permitted a fast and specific detection of target cysts in sediment samples. We also detected dinoflagellate and raphidophyte cysts at concentrations not detectable by microscopic methods or that are difficult to identify. The results given by molecular and microscopic methods were comparable. However, higher values of positive detection for target cysts were obtained by PCR than with microscopy. Some taxa were detected in 100% of the samples using PCR, while others were only found in 10% of the samples. The data obtained in this study showed that the PCR-based method is a valid tool for cyst identification in marine sediments. (literal)
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