http://www.cnr.it/ontology/cnr/individuo/prodotto/ID49780
Detection of microalgal resting cysts in European coastal sediments using a PCR-based assay (Articolo in rivista)
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- Label
- Detection of microalgal resting cysts in European coastal sediments using a PCR-based assay (Articolo in rivista) (literal)
- Anno
- 2010-01-01T00:00:00+01:00 (literal)
- Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#doi
- 10.1016/j.dsr2.2009.09.010 (literal)
- Alternative label
- Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#autori
- Penna A; Battocchi C; Garces E; Angles S; Cucchiari E; Totti C; Kremp A; Satta C; Giacobbe MG; Bravo I; Bastianini M (literal)
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- Note
- ISI Web of Science (WOS) (literal)
- Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#affiliazioni
- Penna, Antonella
Dipartimento di Scienze Biomolecolari, Università di Urbino, Viale Trieste 296, 61100 Pesaro, Italy
Battocchi, Cecilia
Dipartimento di Scienze Biomolecolari, Università di Urbino, Viale Trieste 296, 61100 Pesaro, Italy
Garces, Esther
CSIC, ICM, Inst Marine Sci, Barcelona 08002, Spain
Angles, Silvia
CSIC, ICM, Inst Marine Sci, Barcelona 08002, Spain
Cucchiari, Emelina
Univ Politecn Marche, Dept Marine Sci, Ancona, Italy
Totti, Cecilia
Univ Politecn Marche, Dept Marine Sci, Ancona, Italy
Kremp, Anke
Tvarminne Zoological Station, University of Helsinki, 10900 Hanko, Finland
Satta, Cecilia
Dip. Botanica ed Ecologia Vegetale, University of Sassari, 07100 Sassari, Italy
Giacobbe, Maria Grazia
CNR-IAMC, Messina
Bravo, Isabel
Instituto Espan~ol de Oceanograf?´a, Apdo. 1552, 36200 Vigo, Spain
Bastianini, Mauro
CNR-ISMAR, Venezia (literal)
- Titolo
- Detection of microalgal resting cysts in European coastal sediments using a PCR-based assay (literal)
- Abstract
- A PCR-based assay was developed and applied to sediment and sediment trap samples for the detection of different cysts belonging to dinoflagellates and raphidophytes in European coastal areas. Oligonucleotide primers were designed based on the ITS-5.8S and LSU ribosomal gene sequences. The specificity and sensitivity of the PCR assay were assessed using genomic DNA from clonal cultures, plasmid copy number of cloned target sequences, as well as from sediment samples. Qualitative PCR determinations of different cysts in sediment and sediment trap samples were compared to taxonomic examinations by light microscopy. This molecular methodology permitted a fast and specific detection of target cysts in sediment samples. We also detected dinoflagellate and raphidophyte cysts at concentrations not detectable by microscopic methods or that are difficult to identify. The results given by molecular and microscopic methods were comparable. However, higher values of positive detection for target cysts were obtained by PCR than with microscopy. Some taxa were detected in 100% of the samples using PCR, while others were only found in 10% of the samples. The data obtained in this study showed that the PCR-based method is a valid tool for cyst identification in marine sediments. (literal)
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