Heterochromatin of the scarab beetle, Bubas bison (Coleoptera : Scarabaeidae) II. Evidence for AT-rich compartmentalization and a high amount of rDNA copies (Articolo in rivista)

Type

• Articolo in rivista (Classe)
• Prodotto della ricerca (Classe)

Label

• Heterochromatin of the scarab beetle, Bubas bison (Coleoptera : Scarabaeidae) II. Evidence for AT-rich compartmentalization and a high amount of rDNA copies (Articolo in rivista) (literal)

Anno

• 2006-01-01T00:00:00+01:00 (literal)

Alternative label

• Colomba M. (a), Vitturi R. (b), Libertini A. (c), Gregorini A. (d), Zunino M. (a) (2006)
  Heterochromatin of the scarab beetle, Bubas bison (Coleoptera : Scarabaeidae) II. Evidence for AT-rich compartmentalization and a high amount of rDNA copies
  in Micron (1993)
  (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#autori

• Colomba M. (a), Vitturi R. (b), Libertini A. (c), Gregorini A. (d), Zunino M. (a) (literal)

Pagina inizio

• 47 (literal)

Pagina fine

• 51 (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#altreInformazioni

• doi:10.1016/j.micron.2005.06.004 (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#numeroVolume

• 37 (literal)

Rivista

• Micron (Rivista)

Note

• ISI Web of Science (WOS) (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#affiliazioni

• (a)Istituto di Ecologia e Biologia Ambientale, Università di Urbino ‘Carlo Bo’, Via Sasso 75, 61029 Urbino (PU), Italy (b)Dipartimento di Biologia Animale, Università di Palermo, Via Archirafi 18, 90123 Palermo, Italy (c)Istituto di Scienze Marine, CNR, Riva 7 Martiri 1364/a, 30122 Venezia, Italy (d)Istituto di Psicologia, Università di Urbino ‘Carlo Bo’, Via Zeppi, 61029 Urbino (PU), Italy (literal)

Titolo

• Heterochromatin of the scarab beetle, Bubas bison (Coleoptera : Scarabaeidae) II. Evidence for AT-rich compartmentalization and a high amount of rDNA copies (literal)

Abstract

• An unexpected result arising from a previous characterization of the scarab beetle Bubas bison (Coleoptera: Scarabaeidae) heterochromatin was its unusual homogeneous reaction to different staining methods. In particular, silver stainability of heterochromatic ends of all chromosomes prevented identification of the number of rDNA transcriptionally active regions. Data formerly obtained using silver impregnation (Ag-NOR), C- G- and DAPI banding are here improved and completed by application of CMA3 staining and rDNA FISH with the aim to investigate heterochromatin base composition and locate rDNA regions with respect to NOR-associated heterochromatin. Our results show that B. bison has a high amount of heterochromatin (almost 50%) and that - as revealed by rDNA FISH - major rRNA genes are spread over the heterochromatic telomeric regions of eight chromosomes, thus suggesting that only a portion, although consistent, of total heterochromatin is associated with ribosomal clusters. Moreover, DAPI-positive (AT-specific) and CMA3-negative (GC-specific) reactions of heterochromatic DNA confirm its AT-rich composition. Finally, possible explanations for the bright DAPI-fluorescence of both heterochromatin and rDNA sequences are discussed. (literal)

Prodotto di

• Processi ambientali e valutazione della qualità negli ambienti di transizione (TA.P05.015.003) (Modulo)
• Marine science institute (ISMAR) (Istituto)

Autore CNR

• ANGELO LIBERTINI (Unità di personale interno)

Insieme di parole chiave

• Parole chiave di "Heterochromatin of the scarab beetle, Bubas bison (Coleoptera : Scarabaeidae) II. Evidence for AT-rich compartmentalization and a high amount of rDNA copies" (Insieme di parole chiave)

Incoming links:

Prodotto

• Marine science institute (ISMAR) (Istituto)
• Processi ambientali e valutazione della qualità negli ambienti di transizione (TA.P05.015.003) (Modulo)

Autore CNR di

• ANGELO LIBERTINI (Unità di personale interno)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#rivistaDi

• Micron (Rivista)

Insieme di parole chiave di

• Parole chiave di "Heterochromatin of the scarab beetle, Bubas bison (Coleoptera : Scarabaeidae) II. Evidence for AT-rich compartmentalization and a high amount of rDNA copies" (Insieme di parole chiave)