http://www.cnr.it/ontology/cnr/individuo/prodotto/ID48911
High fraction of dead and inactive bacteria in coastal marine sediments: comparison of protocols and ecological significance (Articolo in rivista)
- Type
- Label
- High fraction of dead and inactive bacteria in coastal marine sediments: comparison of protocols and ecological significance (Articolo in rivista) (literal)
- Anno
- 2002-01-01T00:00:00+01:00 (literal)
- Alternative label
Luna G.M. 1, Manini E. 2, Danovaro R. 1 (2002)
High fraction of dead and inactive bacteria in coastal marine sediments: comparison of protocols and ecological significance
in Applied and environmental microbiology (Print)
(literal)
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- Luna G.M. 1, Manini E. 2, Danovaro R. 1 (literal)
- Pagina inizio
- Pagina fine
- Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#altreInformazioni
- In this study we estimated the fraction of bacteria actively contributing to bacterial carbon production and calculated the actual turnover rate of actively growing bacteria in different marine sediments. To do this, we compared two different protocols. Finally, we conducted microcosm experiments to estimate the fraction of dormant bacteria that was inducible to active metabolism after organic and inorganic nutrient supply, to quantify the \"dormant-reactived\" bacterial fraction. Impact factor: 3.691 (literal)
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- Rivista
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- Pubblicazione scientifica su rivista internazionale (JCR) (literal)
- Note
- ISI Web of Science (WOS) (literal)
- Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#affiliazioni
- 1. Università Politecnica delle Marche
2. CNR-ISMAR (literal)
- Titolo
- High fraction of dead and inactive bacteria in coastal marine sediments: comparison of protocols and ecological significance (literal)
- Abstract
- It is now universally recognized that only a portion of aquatic bacteria
is actively growing, but quantitative information on the fraction of
living vs dormant or dead bacteria in marine sediments is completely
lacking. We compared different protocols for the determination of the
dead, dormant and active bacterial fractions in two different marine
sediments and at different depths into the sediment core. Bacterial counts
ranged from 1.5±0.2 to 53.1±16.0 ´108 cells g-1 in sandy and muddy
sediments, respectively. Bacteria displaying intact membrane (Live
Bacterial Cells) accounted for 26-30% of total bacterial counts, while
dead cells represented the most abundant fraction (70-74%). Among Living
Bacterial Cells, Nucleoid Containing Cells represented only 4% of total
bacterial counts, indicating that only a very limited fraction of
bacterial assemblage was actively growing. Nucleoid-containing cells
increased with increasing sediment organic content. The number of bacteria
responsive to antibiotic treatment (Direct Viable Count, range 0.3-4.8% of
the total bacterial number) was significantly lower than Nucleoid
Containing Cells counts. An experiment of nutrient enrichment to stimulate
a response of the dormant bacterial fraction determined a significant
increase of Nucleoid Containing Cells. After nutrient enrichment, a large
fraction of dormant bacteria (6-11% of total bacterial number)
was reactivated. Bacterial turnover rates estimated ranged from 0.01 to
0.1 d-1, but were 50-80 times higher when only the fraction of active
bacteria was considered (on average 3.2 d-1). Our results suggest that the
fraction of active bacteria in marine sediments is controlled by nutrient
supply and availability and that their turnover rates is at least one
order of magnitude higher than previously reported. (literal)
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