http://www.cnr.it/ontology/cnr/individuo/prodotto/ID4881

Identification of a caspase-derived N-terminal tau fragment in cellular and animal Alzheimer's disease models (Articolo in rivista)

Type

Articolo in rivista (Classe)
Prodotto della ricerca (Classe)

Label

Identification of a caspase-derived N-terminal tau fragment in cellular and animal Alzheimer's disease models (Articolo in rivista) (literal)

Anno

2008-01-01T00:00:00+01:00 (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#doi

10.1016/j.mcn.2008.03.011 (literal)

Alternative label

Corsetti b; G. Amadoro a, b; A. Gentile a; S. Capsoni c; M.T. Ciotti c; M.T. Cencioni e; A. Atlante d; N. Canu a, b; T.T. Rohn f; A. Cattaneo b; P. Calissano a, b (2008)
Identification of a caspase-derived N-terminal tau fragment in cellular and animal Alzheimer's disease models
in Molecular and cellular neurosciences (Print)
(literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#autori

Corsetti b; G. Amadoro a, b; A. Gentile a; S. Capsoni c; M.T. Ciotti c; M.T. Cencioni e; A. Atlante d; N. Canu a, b; T.T. Rohn f; A. Cattaneo b; P. Calissano a, b (literal)

Pagina inizio

381 (literal)

Pagina fine

392 (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#altreInformazioni

impact factor 3.861 (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#numeroVolume

38 (literal)

Rivista

Molecular and cellular neurosciences (Rivista)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#numeroFascicolo

3 (literal)

Note

ISI Web of Science (WOS) (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#affiliazioni

a Institute of Neurobiology and Molecular Medicine, CNR, Via del Fosso di Fiorano 64-65, 00143 Rome, Italy; b European Brain Research Institute (EBRI), Via del Fosso di Fiorano 64-65, 00143 Rome, Italy; c Lay Line Genomics S.p.A. c/o EBRI, Via del Fosso di Fiorano 64-65, 00143 Rome, Italy; d Institute of Biomembranes and Bioenergetics, CNR, Via Amendola 165/A, 70126 Bari, Italy; e Laboratory of Neuroimmunology, Fondazione Santa Lucia, Rome, Italy; f Department of Biology, Boise State University, Boise, ID 83725, USA (literal)

Titolo

Identification of a caspase-derived N-terminal tau fragment in cellular and animal Alzheimer's disease models (literal)

Abstract

Biochemical modifications of tau proteins have been proposed to be among the earliest neurobiological changes in Alzheimer's disease (AD) and correlate better with cognitive symptoms than do beta-amyloid plaques. We have recently reported that adenovirus-mediated overexpression of the NH2 26-230aa tau fragment evokes a potent NMDA-mediated neurotoxic effect in primary neuronal cultures. In order to assess whether such N-terminal tau fragment(s) are indeed produced during apoptosis or neurodegeneration in vivo, we attempted to ascertain their presence in cell and animal models using an anti-tau antibody directed against the N-terminal sequence of human protein located downstream of the caspase(s)-cleavage site DRKD(25)-QGGYTMHQDQ. We provide biochemical evidence that a caspase(s)-cleaved NH2-terminal tau fragment of 20-22 kDa, consistent with the size of the NH2 26-230aa neurotoxic fragment of tau, is generated in vitro in differentiated human SH-SY5Y cells undergoing apoptosis by BDNF withdrawal or following treatment with staurosporine. In addition this NH2-terminally cleaved tau fragment, whose expression correlates with a significant up-regulation of caspase(s) activity, is also specifically detected in vivo in the hippocampus of 15 month-old AD11 transgenic mice, a model in which a progressive AD-like neurodegeneration is induced by the expression of transgenic anti-NGF antibodies. The results support the idea that aberrant activation of caspase(s), following apoptotic stimuli or neurodegeneration insults, may produce one or more toxic NH2 tau fragments, that further contribute to propagate and increase cellular dysfunctions in AD. (literal)

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