Low Density Lipoprotein Misfolding and Amyloidogenesis (Articolo in rivista)

Type
Label
  • Low Density Lipoprotein Misfolding and Amyloidogenesis (Articolo in rivista) (literal)
Anno
  • 2008-01-01T00:00:00+01:00 (literal)
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#doi
  • 10.1096/fj.07-097774 (literal)
Alternative label
  • Tiziana Parasassi1; Marco De Spirito2; Giampiero Mei3; Roberto Brunelli4; Giulia Greco1; Laura Lenzi1; Giuseppe Maulucci2; Eleonora Nicolai3; Massimiliano Papi2; Giuseppe Arcovito2; Silvio C. Tosatto5; Fulvio Ursini6 (2008)
    Low Density Lipoprotein Misfolding and Amyloidogenesis
    in The FASEB journal
    (literal)
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#autori
  • Tiziana Parasassi1; Marco De Spirito2; Giampiero Mei3; Roberto Brunelli4; Giulia Greco1; Laura Lenzi1; Giuseppe Maulucci2; Eleonora Nicolai3; Massimiliano Papi2; Giuseppe Arcovito2; Silvio C. Tosatto5; Fulvio Ursini6 (literal)
Pagina inizio
  • 2350 (literal)
Pagina fine
  • 2356 (literal)
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#numeroVolume
  • 22 (literal)
Rivista
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#numeroFascicolo
  • 7 (literal)
Note
  • PubMe (literal)
  • ISI Web of Science (WOS) (literal)
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#affiliazioni
  • 1 Istituto di Neurobiologia e Medicina Molecolare, CNR, Rome, Italy; 2 Istituto di Fisica, Facoltà di Medicina e Chirurgia, Università Cattolica del Sacro Cuore, Rome, Italy; 3 Dipartimento di Medicina Sperimentale e Scienze Biochimiche, Università di Roma \"Tor Vergata,\" Rome, Italy; 4 Dipartimento di Scienze Ginecologiche, Perinatologia e Puericultura, Università di Roma \"La Sapienza,\" Rome, Italy; 5 Dipartimento di Biologia and Centro per le Biotecnologie Innovative (CRIBI), Università di Padova, Italy 6 Dipartimento di Chimica Biologica, Università di Padova, Italy (literal)
Titolo
  • Low Density Lipoprotein Misfolding and Amyloidogenesis (literal)
Abstract
  • In early atherogenesis, subendothelial retention of lipidic droplets is associated with an inflammatory response-to-injury, culminating in the formation of foam cells and plaque. Low density lipoprotein (LDL) is the main constituent of subendothelial lipidic droplets. The process is believed to occur following LDL modification. Searching for a modified LDL in plasma, electronegative LDL(-) was identified and found to be associated with major risk biomarkers. The apoprotein in LDL(-) is misfolded and we show here that this modification primes the aggregation of native LDL, conforming to the typical pattern of protein amyloidogenesis. After a lag phase, whose length depends on LDL(-) concentration, light scattering and atomic force microscopy reveal early exponential growth of intermediate globules, which evolve into fibrils. These globules are remarkably similar to subendothelial droplets in atheromatous lesions and different from those produced by oxidation or biochemical manipulation. During aggregation, ellipticity and tryptophan fluorescence measurements reveal a domino-style spread of apoprotein misfolding from LDL(-) to all of the LDL. Computational analysis of the apoprotein primary sequence predicts an unstable, aggregation-prone domain in the regulatory alpha2 region. Apoprotein misfolding well represents a LDL modification able to transform this cholesterol carrier into a trigger for a response-to-injury in the artery wall. (literal)
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