http://www.cnr.it/ontology/cnr/individuo/prodotto/ID48496
Immunodetection of Proteins in Ancient Paint Media (Articolo in rivista)
- Type
- Label
- Immunodetection of Proteins in Ancient Paint Media (Articolo in rivista) (literal)
- Anno
- 2010-01-01T00:00:00+01:00 (literal)
- Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#doi
- 10.1021/ar900279d (literal)
- Alternative label
Cartechini, Laura; Vagnini, Manuela; Palmieri, Melissa; Pitzurra, Lucia; Mello, Tommaso; Mazurek, Joy; Chiari, Giacomo (2010)
Immunodetection of Proteins in Ancient Paint Media
in Accounts of chemical research
(literal)
- Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#autori
- Cartechini, Laura; Vagnini, Manuela; Palmieri, Melissa; Pitzurra, Lucia; Mello, Tommaso; Mazurek, Joy; Chiari, Giacomo (literal)
- Pagina inizio
- Pagina fine
- Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#numeroVolume
- Rivista
- Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#note
- DOI: 10.1021/ar900279d (literal)
- Note
- ISI Web of Science (WOS) (literal)
- Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#affiliazioni
- [Cartechini, Laura] Univ Perugia, Dipartimento Chim, CNR, Ist Sci & Tecnol Mol, I-06123 Perugia, Italy.
[Pitzurra, Lucia] Univ Perugia, Dipartimento Med Sperimentale & Sci Biochim, I-06126 Perugia, Italy.
[Mello, Tommaso] Univ Florence, Dipartimento Fisiopatol Clin, I-50134 Florence, Italy.
[Mazurek, Joy; Chiari, Giacomo] Getty Conservat Inst, Los Angeles, CA 90049 USA. (literal)
- Titolo
- Immunodetection of Proteins in Ancient Paint Media (literal)
- Abstract
- Diagnostic immunology is a powerful tool, widely used in clinical and
biochemical laboratories for detecting molecules. In recent years, the
technique has been adaptated to materials sciences as a result of the
extensive advances achieved in immunology. Today, many companies supply
custom antibodies as well as new high-performance bioprobes for
virtually any use.
The idea of using immunodetection in the field of conservation science
is not new. This analytical methodology is, in fact, particularly
attractive for investigating biopolymers in painting materials; it is
highly sensitive and selective with respect to the biological source of
the target molecules. Among biopolymers, proteins have been widely used
in the past as painting binders, adhesives, and additives in coating
layers. An accurate assessment of these materials is necessary to
obtain deeper insights into an artist's technique as well as to design
proper restoration and conservation methods. In spite of the diagnostic
potential offered by immunodetection-based techniques, some analytical
drawbacks had, until recently, limited their use in routine
applications in conservation science.
In this Account, we highlight the most important results achieved in
our research on the development of analytical methodologies based on
the use of enzyme-linked immunosorbent assay (ELISA) and
immuno-fluorescence microscopy (IFM) techniques for the highly
sensitive and specific identification of proteins in artistic and
archeological materials. ELISA and IFM offer two alternative analytical
routes to this final goal: ELISA provides a fast, cost-effective,
quantitative analysis of microsamples put in solution, whereas IFM
combines the immunodetection of the targeted molecules with the
characterization of their spatial distribution. The latter approach is
of great value in the stratigraphic investigation of paintings. We
discuss the limits and strengths of these methodologies in the context
of the complex matrixes usually found in the investigated materials and
the prolonged aging that they have undergone.
Immunology is a relatively new technique in conservation science,
providing a rich new field for innovation. We see two areas that are
particularly ripe for future contributions. The commercial manufacture
of antibodies specifically tailored for use in cultural heritage
studies holds enormous potential. Moreover, the need for further
refinement of detection systems in immuno-fluorescence techniques,
especially the suppression of the autofluorescence background in
painting materials, offers an abundance of opportunities for
researchers. Immunology is a relatively new technique in conservation
science, providing a rich new field for innovation. (literal)
- Prodotto di
- Autore CNR
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