Identification of proteins in painting cross-sections by immunofluorescence microscopy (Articolo in rivista)

Type
Label
  • Identification of proteins in painting cross-sections by immunofluorescence microscopy (Articolo in rivista) (literal)
Anno
  • 2008-01-01T00:00:00+01:00 (literal)
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#doi
  • 10.1007/s00216-008-2041-9 (literal)
Alternative label
  • Vagnini, M.; Cartechini, L.; Miliani, C.; Brunetti, B. G.; Sgamellotti, A. (2008)
    Identification of proteins in painting cross-sections by immunofluorescence microscopy
    in Analytical & bioanalytical chemistry (Print)
    (literal)
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#autori
  • Vagnini, M.; Cartechini, L.; Miliani, C.; Brunetti, B. G.; Sgamellotti, A. (literal)
Pagina inizio
  • 57 (literal)
Pagina fine
  • 64 (literal)
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#numeroVolume
  • 392 (literal)
Rivista
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#note
  • DOI: 10.1007/s00216-008-2041-9 (literal)
Note
  • ISI Web of Science (WOS) (literal)
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#affiliazioni
  • [Vagnini, M.; Brunetti, B. G.; Sgamellotti, A.] Centro SMAArt, Dipartimento di Chimica, Università di Perugia, via Elce di Sotto 8, 06123 Perugia, Italy [Pitzurra, L.] Centro SMAArt, Dipartimento di Medicina Sperimentale e Scienze Biochimiche, Università di Perugia, Via del Giochetto, 06123 Perugia, Italy [Cartechini, L.; Miliani, C.] Istituto di Scienze e Tecnologie Molecolari, CNR, via Elce di Sotto 8,06123 Perugia, Italy (literal)
Titolo
  • Identification of proteins in painting cross-sections by immunofluorescence microscopy (literal)
Abstract
  • Immunofluorescence microscopy offers a highly specific analytical tool for unambiguous recognition and mapping of proteins in complex matrices. In the present work, the analytical potentials of immunofluorescence microscopy have been exploited to provide recognition of proteinaceous binders in painting cross-sections. An optimised analytical protocol is proposed for the identification of ovalbumin and of bovine serum albumin as markers of egg white and casein, respectively. The study has been carried out on laboratory model samples simulating both easel and mural paintings. The obtained results demonstrated the effectiveness of the method, suggesting the potential future use of immunofluorescence microscopy as a routine diagnostic tool in conservation science. Possible developments of the proposed methodology in order to improve the specificity of the method and its detection sensitivity are presented and discussed. (literal)
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