http://www.cnr.it/ontology/cnr/individuo/prodotto/ID48311
Identification of proteins in painting cross-sections by immunofluorescence microscopy (Articolo in rivista)
- Type
- Label
- Identification of proteins in painting cross-sections by immunofluorescence microscopy (Articolo in rivista) (literal)
- Anno
- 2008-01-01T00:00:00+01:00 (literal)
- Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#doi
- 10.1007/s00216-008-2041-9 (literal)
- Alternative label
Vagnini, M.; Cartechini, L.; Miliani, C.; Brunetti, B. G.; Sgamellotti, A. (2008)
Identification of proteins in painting cross-sections by immunofluorescence microscopy
in Analytical & bioanalytical chemistry (Print)
(literal)
- Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#autori
- Vagnini, M.; Cartechini, L.; Miliani, C.; Brunetti, B. G.; Sgamellotti, A. (literal)
- Pagina inizio
- Pagina fine
- Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#numeroVolume
- Rivista
- Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#note
- DOI: 10.1007/s00216-008-2041-9 (literal)
- Note
- ISI Web of Science (WOS) (literal)
- Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#affiliazioni
- [Vagnini, M.; Brunetti, B. G.; Sgamellotti, A.] Centro SMAArt, Dipartimento di Chimica, Università di Perugia, via Elce di Sotto 8, 06123 Perugia, Italy
[Pitzurra, L.] Centro SMAArt, Dipartimento di Medicina Sperimentale e Scienze Biochimiche, Università di Perugia, Via del Giochetto, 06123 Perugia, Italy
[Cartechini, L.; Miliani, C.] Istituto di Scienze e Tecnologie Molecolari, CNR, via Elce di Sotto 8,06123 Perugia, Italy (literal)
- Titolo
- Identification of proteins in painting cross-sections by immunofluorescence microscopy (literal)
- Abstract
- Immunofluorescence microscopy offers a highly specific analytical tool
for unambiguous recognition and mapping of proteins in complex
matrices. In the present work, the analytical potentials of
immunofluorescence microscopy have been exploited to provide
recognition of proteinaceous binders in painting cross-sections. An
optimised analytical protocol is proposed for the identification of
ovalbumin and of bovine serum albumin as markers of egg white and
casein, respectively. The study has been carried out on laboratory
model samples simulating both easel and mural paintings. The obtained
results demonstrated the effectiveness of the method, suggesting the
potential future use of immunofluorescence microscopy as a routine
diagnostic tool in conservation science. Possible developments of the
proposed methodology in order to improve the specificity of the method
and its detection sensitivity are presented and discussed. (literal)
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- Autore CNR
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