http://www.cnr.it/ontology/cnr/individuo/prodotto/ID4807
Electroporation of skeletal muscle induces danger signal release and antigen-presenting cell recruitment independently of DNA vaccine administration. (Articolo in rivista)
- Type
- Label
- Electroporation of skeletal muscle induces danger signal release and antigen-presenting cell recruitment independently of DNA vaccine administration. (Articolo in rivista) (literal)
- Anno
- 2008-01-01T00:00:00+01:00 (literal)
- Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#doi
- 10.1517/14712590802472606 (literal)
- Alternative label
- Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#autori
- Chiarella P.1; Massi E.1; De Robertis M.1; Sibilio A.; Parrella P; Fazio V.M.2; Signori E.1 (literal)
- Pagina inizio
- Pagina fine
- Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#numeroVolume
- Rivista
- Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#numeroFascicolo
- Note
- ISI Web of Science (WOS) (literal)
- Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#affiliazioni
- 1 = CNR, Institute of Neurobiology and Molecular Medicine, Research Area of Tor Vergata, Rome, Italy;
2 = Laboratory of Molecular Medicine and Biotechnology, CIR, Campus Bio-Medico University of Rome, Via Alvaro del Portillo 21 - 00128 Rome, Italy (literal)
- Titolo
- Electroporation of skeletal muscle induces danger signal release and antigen-presenting cell recruitment independently of DNA vaccine administration. (literal)
- Abstract
- Background : Plasmid DNA vaccination combined with electroporation (EP)
provides a promising approach for the prevention of infectious diseases and
for cancer immunotherapy. This technology has been described as being
effective in activating humoural and cellular immune response in the host
as well as in enhancing expression of the encoded antigen. Several reports
showed EP has adjuvant-like properties when combined with plasmid DNA
injection although the effect in the absence of DNA has not been investigated.
Objective : The aim of this study is to clarify whether the application of EP
alone to the skeletal muscle is able to recruit and trigger cells involved in
antigen presentation and immune response. Methods : Mouse skeletal muscle
treated by EP were observed and processed for clinical, histological and
immunohistochemistry analysis at different time points. Results : We demonstrate
that EP induces transient morphological changes in the muscle
with early production of endogenous cytokines responsible for signalling
danger at the local level. Moreover, it causes the recruitment of inflammatory
cells independently of the DNA injection and the activation of a danger
pro-inflammatory pathway, resulting in T-lymphocyte migration. Conclusions :
Our data indicate EP by itself is able to recruit and trigger cells involved in
antigen presentation and immune response; hence, the idea that EP has
adjuvant-like properties owing to a moderate tissue injury and generation
of a pro-inflammatory context with cytokine release that enhances the
immune response. We suggest EP may be of practical use in clinical protocols,
contributing to the development of DNA vaccination strategies. (literal)
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- Autore CNR
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