Molecular and Cellular Determinants of Cell-to-Cell Transmission of HCV In Vitro. (Articolo in rivista)

Type
Label
  • Molecular and Cellular Determinants of Cell-to-Cell Transmission of HCV In Vitro. (Articolo in rivista) (literal)
Anno
  • 2007-01-01T00:00:00+01:00 (literal)
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#doi
  • 10.1002/jmv.20947 (literal)
Alternative label
  • Valli M.B.1,2; Crema A.1; Lanzilli G.1; Serafino A.1; Bertolini L.1; Ravagnan G.3; Ponzetto A.4; Menzo S.5; Clementi M.6; Carloni G.1 (2007)
    Molecular and Cellular Determinants of Cell-to-Cell Transmission of HCV In Vitro.
    in Journal of medical virology (Print)
    (literal)
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#autori
  • Valli M.B.1,2; Crema A.1; Lanzilli G.1; Serafino A.1; Bertolini L.1; Ravagnan G.3; Ponzetto A.4; Menzo S.5; Clementi M.6; Carloni G.1 (literal)
Pagina inizio
  • 1491 (literal)
Pagina fine
  • 1499 (literal)
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#altreInformazioni
  • Impact factor = 2.779 (literal)
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#numeroVolume
  • 79 (literal)
Rivista
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#numeroFascicolo
  • 10 (literal)
Note
  • ISI Web of Science (WOS) (literal)
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#affiliazioni
  • 1 = Institute of Neurobiology and Molecular Medicine, National Research Council (CNR), Rome, Italy; 2 = Institute of Experimental Zooprofilaxis, Umbria-Marche, Macerata, Italy; 3 = Department of Environmental Science CA' Foscari, University of Venezia, Venezia, Italy; 4 = Department of Internal Medicine, Torino University, Torino, Italy; 5 = Institute of Microbiology, University of Ancona, Ancona, Italy; 6 = Laboratory of Microbiology, Vita-Salute San Raffaele University, San Raffaele Institute, Milan, Italy. (literal)
Titolo
  • Molecular and Cellular Determinants of Cell-to-Cell Transmission of HCV In Vitro. (literal)
Abstract
  • It was reported previously that HCV can be transmitted from persistently infected human bone-marrow-derived B-lymphoblastoid cells (TO.FE(HCV)) to human hepatoma cells by cell-to-cell contact. The present study confirms and characterize further such type of HCV infection in vitro. TO.FE(HCV) cells were co-cultured with 2.2.15 hepatoma cells, that are not susceptible to cell-free infection by sera containing HCV of 1b genotype. By this co-cultivation system it was demonstrated that HCV transmission to recipient cells requires de novo virus RNA replication. Several factors may favor HCV-transmission, evidence is provided that TO.FE(HCV) cells were able to select HCV-quasispecies. 5'-UTR and core sequence analysis revealed differences in the HCV-quasispecies composition in serum inoculum and in infected TO.FE B-cells at 4 months post-inoculation. It is considered that the latter may be more successful in replicating HCV in vitro and used to express surface molecules which may be involved in cell-to-cell contact. In TO.FE(HCV) cells replicate distinct, or few close related, HCV-variants correlated with those of serum inoculum. Comparative analysis of tetra-spans and integrins expression (literal)
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