Toxicological profile of cereulide, the Bacillus cereus emetic toxin, in functional assays with human, animal and bacterial cells. (Articolo in rivista)

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  • Toxicological profile of cereulide, the Bacillus cereus emetic toxin, in functional assays with human, animal and bacterial cells. (Articolo in rivista) (literal)
Anno
  • 2007-01-01T00:00:00+01:00 (literal)
Alternative label
  • Andersson M.A. a, Hakulinen P, b, Honkalampi-Hamalainen U. c, Hoornstra D. a, Lhuguenot J.C. d, Maki-Paakkanen J. b, Savolainen M. e, Severin I. d, Stammati A.L. f, Turco L. f, Weber A. c, von Wright A. c, Zucco F. g, Salkinoja-Salonen M. a (2007)
    Toxicological profile of cereulide, the Bacillus cereus emetic toxin, in functional assays with human, animal and bacterial cells.
    in Toxicon (Oxf.)
    (literal)
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  • Andersson M.A. a, Hakulinen P, b, Honkalampi-Hamalainen U. c, Hoornstra D. a, Lhuguenot J.C. d, Maki-Paakkanen J. b, Savolainen M. e, Severin I. d, Stammati A.L. f, Turco L. f, Weber A. c, von Wright A. c, Zucco F. g, Salkinoja-Salonen M. a (literal)
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  • 351 (literal)
Pagina fine
  • 367 (literal)
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  • 49 (literal)
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  • ISI Web of Science (WOS) (literal)
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  • a Department of Applied Chemistry and Microbiology, University of Helsinki, Biocenter, P.O. Box 56, Fi 00014 Helsinki University, Finland; b National Public Health Institute, P.O. Box 95, Fi-70701 Kuopio, Finland; c Institute of Applied Biotechnology, University of Kuopio, P.O. Box 1627, FIN-70211 Kuopio, Finland; d Food Toxicology Laboratory, Université de Bourgogne, Campus Universitaire, 1, Esplanade Erasme, 21 000 Dijon, France; e Oy Keskuslaboratorio – Centrallaboratorium Ab, KCL Science and Consulting, P.O. Box 70 Fi-02151 Espoo, Finland; f Istituto Superiore di Sanità, Department of Environment and Primary Prevention, Viale Regina Elena, 299, 00161 Roma, Italy; g Istituto di Neurobiologia e Medicina Molecolare, CNR, Via Fosso del Fiorano 61, 00143 Rome, Italy (literal)
Titolo
  • Toxicological profile of cereulide, the Bacillus cereus emetic toxin, in functional assays with human, animal and bacterial cells. (literal)
Abstract
  • Some strains of the endospore-forming bacterium Bacillus cereus produce a heat-stable ionophoric peptide, cereulide, of high human toxicity. We assessed cell toxicity of cereulide by measuring the toxicities of crude extracts of cereulide producing and non-producing strains of B. cereus, and of pure cereulide, using cells of human, animal and bacterial origins. Hepatic cell lines and boar sperm, with cytotoxicity and sperm motility, respectively, as the end points, were inhibited by 1 nM of cereulide present as B. cereus extract. RNA synthesis and cell proliferation in HepG2 cells was inhibited by 2 nM of cereulide. These toxic effects were explainable by the action of cereulide as a high-affinity mobile K+ carrier. Exposure to cereulide containing extracts of B. cereus caused neither activation of CYP1A1 nor genotoxicity (comet assay, micronucleus test) at concentrations below those that were cytotoxic (0.6 nM cereulide). Salmonella typhimurium reverse mutation (Ames) test was negative. Exposure of Vibrio fischeri to extracts of B. cereus caused stimulated luminescence up to 600%, independent on the presence of cereulide, but purified cereulide inhibited the luminescence with an IC50% (30 min) of 170 nM. Thus the luminescence-stimulating B. cereus substance(s) masked the toxicity of cereulide in B. cereus extracts to V. fischeri. (literal)
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